Anti-Cytochrome P450 2B1 + 2B2 antibody [b/e3 (9.14)] (ab22719)
![Anti-Cytochrome P450 2B1 + 2B2 antibody [b/e3 (9.14)] (ab22719)](https://www.abcam.com/ps/products/22/ab22719/Images/ab22719-196681-anti-cytochrome-p450-2b1-2b2-antibody-b-e3-914-immunoprecipitation.jpg "Anti-Cytochrome P450 2B1 + 2B2 antibody [b/e3 (9.14)] (ab22719)")
Key features and details
- Mouse monoclonal [b/e3 (9.14)] to Cytochrome P450 2B1 + 2B2
- Suitable for: IP
- Reacts with: Mouse
- Isotype: IgG1
Overview
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Product name
Anti-Cytochrome P450 2B1 + 2B2 antibody [b/e3 (9.14)]
See all Cytochrome P450 2B1 + 2B2 primary antibodies -
Description
Mouse monoclonal [b/e3 (9.14)] to Cytochrome P450 2B1 + 2B2 -
Host species
Mouse -
Specificity
This product does not react with Rat CYP 1A1, 1A2, 2A1, 2C6, 2C7, 2C11, 4A2 and 4A3 -
Tested applications
Suitable for: IPmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Rat liver cytochrome P450 2B1 and 2B2.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS
Buffer: PBS -
Concentration information loading... -
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
b/e3 (9.14) -
Isotype
IgG1 -
Research areas
Images
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Immunoprecipitation - Anti-Cytochrome P450 2B1 + 2B2 antibody [b/e3 (9.14)] (ab22719)
Cytochrome P450 2B1 + 2B2 was immunoprecipitated using 0.5mg Mouse Liver tissue, 5µg of Mouse monoclonal to Cytochrome P450 2B1 + 2B2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Liver tissue lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab22719.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.
Band: 56kDa; Cytochrome P450 2B1 + 2B2
