Golgi/ER Staining Kit - Cytopainter (ab139485)
Overview
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Product name
Golgi/ER Staining Kit - Cytopainter -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Golgi/ER Staining Kit | Cytopainter (ab139485) provides a rapid and convenient method for visualizing the Golgi and ER within living cells, without requiring lengthy transfection procedures. ab139485 is validated with human cervical carcinoma cell line HeLa, human T-lymphocyte cell line Jurkat, canine kidney cell line MDCK, and human bone osteosarcoma epithelial cell line U2OS. The kit should also be suitable for identifying Golgi body and endoplasmic reticulum perturbing agents and thus can be a useful tool for examining the transport and recycling of molecules from the GA to ER in cellular secretory pathways.
Organelle Reagent III contains a mixture of GA-selective, ER-selective and nucleus-selective dyes suitable for live cell staining. Compared with other commercially available dyes for labeling Golgi bodies, the green dye component of the Reagent III is more faithfully localized to the Golgi apparatus, with minimal staining of the endoplasmic reticulum. The red dye component of the Reagent III stains the endoplasmic reticulum with high fidelity and is specifically designed for use with green fluorescing probes.
Review other dyes and kits for Golgi staining, or the live cell staining fluorescent dyes guide
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Platform
Fluorescence microscope
Properties
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Storage instructions
Please refer to protocols. -
Components 100 tests 10X Assay Buffer 1 1 x 15ml 50X Assay Buffer 2 1 x 1.2ml Organelle Reagent III (lyophilized) 1 vial -
Research areas
Images
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Fluoroescent staining using CytoPainter Golgi/ER Staining Kit (ab139485) This image is courtesy of an Abreview submitted by Edwin Hernandez
CytoPainter Golgi/ER Staining Kit (ab139485) staining live rat astrocyte cells. Cells were seeded on a Ibidi u-slide 18 well flat poly-L-Lysine coated. Cells were washed with 1XAssay Solution, incubated for 30 min at 37º with the Organelle Reagent III, washed 3 times with ice cold medium (DMEM F-12) and incubated again in medium for 30 minutes at 37º.
ER (red), Golgi (green), Nuclear (blue).
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Absorbance and fluorescent emission - blue dyeAbsorbance and fluorescent emission spectra for the blue dye -
Fluorescence image - red dyeFluorescence excitation and emission spectra for the red dye -
Fluorescence image - green dyeFluorescence excitation and emission spectra for the green dye -
Fluoroescent staining using Organelle Reagent IIIab139485 kit staining of MDCK epithelial cells. The ER (red), Golgi (green) and nuclear (blue) dyes highlight their respective subcellular targets with high dependability.