Anti-NF-kB p65 (phospho T254) antibody (ab131100)
Key features and details
- Rabbit polyclonal to NF-kB p65 (phospho T254)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-NF-kB p65 (phospho T254) antibody
See all NF-kB p65 primary antibodies -
Description
Rabbit polyclonal to NF-kB p65 (phospho T254) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human NF-kB p65 conjugated to Keyhole Limpet Haemocyanin (KLH).
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Positive control
- 3T3 cell extract treated with TNF-alpha; HeLa cells
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS is without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
ab131100 was purified by affinity chromatography using epitope specific phosphopeptide. Non phospho specific antibodies were removed by chromatography using non phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-NF-kB p65 (phospho T254) antibody (ab131100) at 1/500 dilution
Lane 1 : 3T3 cell extract, untreated
Lane 2 : 3T3 cell extract treated with TNF-alpha
Predicted band size: 60 kDa
Actual band size : 65 kDa -
Immunohistochemical analysis of NFkB p65(phospho T254) in paraffin-embedded human breast carcinoma tissue,using ab131100 at a dilution of 1/50.
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Immunofluorescence analysis of NFkB p65 (phospho T254)in methanol fixed HeLa cells, using ab131100 at a dilution of 1/100.