Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CCDC98 antibody [EPR6310(2)] (ab139191)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6310(2)] to CCDC98
  • Suitable for: WB, ICC/IF, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-CCDC98 antibody [EPR6310(2)]
    See all CCDC98 primary antibodies

  • Description

    Rabbit monoclonal [EPR6310(2)] to CCDC98

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human CCDC98 aa 350-450 (C terminal). The exact sequence is proprietary.
    Database link: Q6UWZ7

  • Positive control

    • HeLa, MCF7, 293T and Jurkat whole cell lysate (ab7899); HeLa cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20ºC.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR6310(2)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-CCDC98 antibody [EPR6310(2)] (ab139191)
    Western blot - Anti-CCDC98 antibody [EPR6310(2)] (ab139191)
    All lanes : Anti-CCDC98 antibody [EPR6310(2)] (ab139191) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 47 kDa

  • Flow Cytometry - Anti-CCDC98 antibody [EPR6310(2)] (ab139191)
    Flow Cytometry - Anti-CCDC98 antibody [EPR6310(2)] (ab139191)
    Overlay histogram showing HeLa cells stained with ab139191 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab139191, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Immunocytochemistry/ Immunofluorescence - Anti-CCDC98 antibody [EPR6310(2)] (ab139191)
    Immunocytochemistry/ Immunofluorescence - Anti-CCDC98 antibody [EPR6310(2)] (ab139191)
    Immunofluorescent analysis of HeLa cells labelling CCDC98 with ab139191 at 1/100 dilution.
  • Anti-CCDC98 antibody [EPR6310(2)] (ab139191)
    Anti-CCDC98 antibody [EPR6310(2)] (ab139191)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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