Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Tuesday March 02, 2021

Anti-CCDC98 antibody [EPR6310(2)] (ab139191)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR6310(2)] to CCDC98
Suitable for: WB, ICC/IF, Flow Cyt, IP
Reacts with: Human

Product name

Anti-CCDC98 antibody [EPR6310(2)]
See all CCDC98 primary antibodies
Description

Rabbit monoclonal [EPR6310(2)] to CCDC98
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human CCDC98 aa 350-450 (C terminal). The exact sequence is proprietary.
Database link: Q6UWZ7
Positive control
- HeLa, MCF7, 293T and Jurkat whole cell lysate (ab7899); HeLa cells.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR6310(2)
Isotype

IgG
Research areas

Western blot - Anti-CCDC98 antibody [EPR6310(2)] (ab139191)

All lanes : Anti-CCDC98 antibody [EPR6310(2)] (ab139191) at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Jurkat cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 47 kDa
Flow Cytometry - Anti-CCDC98 antibody [EPR6310(2)] (ab139191)

Overlay histogram showing HeLa cells stained with ab139191 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab139191, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Immunocytochemistry/ Immunofluorescence - Anti-CCDC98 antibody [EPR6310(2)] (ab139191)

Immunofluorescent analysis of HeLa cells labelling CCDC98 with ab139191 at 1/100 dilution.
Anti-CCDC98 antibody [EPR6310(2)] (ab139191)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com