DAF-FM, Fluorescent probe for nitric oxide quantification (ab145297)
Key features and details
- Highly sensitive, photo-stable fluorescent probe for quantification of low concentrations of nitric oxide in solution.
- CAS Number: 254109-20-1
- Purity: > 98%
- Soluble in DMSO
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
DAF-FM, Fluorescent probe for nitric oxide quantification -
Description
Highly sensitive, photo-stable fluorescent probe for quantification of low concentrations of nitric oxide in solution. -
Alternative names
- 3-Amino-4-(N-methylamino)-2', 7'-difluorofluorescein
- 4-Amino-5-methylamino- 2',7'-difluorofluorescein
- Diaminofluorescein-FM
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Biological description
Highly sensitive, photo-stable fluorescent probe for quantification of low concentrations of nitric oxide (NO). More sensitive to nitric oxide (NO) than DAF-2 (ab145269). More photo-stable and less pH sensitive than DAF-2. Not as cell-permeable as the diacetate derivative, DAF-FM DA (ab145295), but it does not need to be activated by cytosolic enzymes. Essentially nonfluorescent until it reacts with NO to form a fluorescent benzotriazole.
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Purity
> 98% -
CAS Number
254109-20-1 -
Chemical structure
Properties
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Excitation
495nm -
Emission
515nm -
Chemical name
3-Amino-4-(N-methylamino)-2',7'-difluorofluorescein -
Molecular weight
412.34 -
Molecular formula
C21H14F2N2O5 -
Storage instructions
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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Source
Synthetic
