Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-EWSR1/EWS antibody [21B1] (ab252829)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [21B1] to EWSR1/EWS
  • Suitable for: WB, IP
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-EWSR1/EWS antibody [21B1]
    See all EWSR1/EWS primary antibodies

  • Description

    Rat monoclonal [21B1] to EWSR1/EWS

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: K562, HeLa, Ramos and F9 whole cell lysate. IP: HeLa and K562 whole cell lysate.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunoprecipitation - Anti-EWSR1/EWS antibody [21B1] (ab252829)
    Immunoprecipitation - Anti-EWSR1/EWS antibody [21B1] (ab252829)

    EWSR1/EWS was immunoprecipitated from K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate with ab252829 at 12.03µg/ml (2μg in 0.35mg lysates). Western blot was performed from the immunoprecipitate using ab252829 at 1/1000 dilution. Goat Anti-Rat IgG (H+L), HRP) (ab205720) was used at 1/5000 dilution.
    Lane 1: K562 whole cell lysate 10µg (Input).
    Lane 2: ab252829 IP in K562 whole cell lysate.
    Lane 3: Rat monoclonal IgG instead of ab252829 in K562 whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 secs.

  • Immunoprecipitation - Anti-EWSR1/EWS antibody [21B1] (ab252829)
    Immunoprecipitation - Anti-EWSR1/EWS antibody [21B1] (ab252829)

    EWSR1/EWS was immunoprecipitated from HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab252829 at 12.03µg/ml (2μg in 0.35mg lysates). Western blot was performed from the immunoprecipitate using ab252829 at 1/1000 dilution. Goat Anti-Rat IgG (H+L), HRP) (ab205720) was used at 1/5000 dilution.
    Lane 1: HeLa whole cell lysate 10µg (Input).
    Lane 2: ab252829 IP in HeLa whole cell lysate.
    Lane 3: Rat monoclonal IgG instead of ab252829 in HeLa whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 secs.

  • Western blot - Anti-EWSR1/EWS antibody [21B1] (ab252829)
    Western blot - Anti-EWSR1/EWS antibody [21B1] (ab252829)
    All lanes : Anti-EWSR1/EWS antibody [21B1] (ab252829) at 0.361 µg/ml

    Lane 1 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
    Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 3 : Ramos (human Burkitt's lymphoma B lymphocyte), whole cell lysate
    Lane 4 : F9 (mouse embryonal carcinoma epithelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

    Predicted band size: 68 kDa


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 15 secs.

  • Anti-EWSR1/EWS antibody [21B1] (ab252829)
    Anti-EWSR1/EWS antibody [21B1] (ab252829)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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