Anti-APE1 antibody [CPTC-APEX1-2] (ab268072)
Key features and details
- Mouse monoclonal [CPTC-APEX1-2] to APE1
- Suitable for: Flow Cyt, IHC-P, Protein Array, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-APE1 antibody [CPTC-APEX1-2]
See all APE1 primary antibodies -
Description
Mouse monoclonal [CPTC-APEX1-2] to APE1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB Human -
Immunogen
Recombinant full length protein within Human APE1 aa 1-318. The exact sequence is proprietary.
Database link: P27695 -
Positive control
- WB: A431, A549, PC3, HAP1, HepG2 cell lysates. ICC/IF: HeLa cells. IHC: human Prostate Carcinoma. Flow Cyt: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from Bioreactor concentrate -
Clonality
Monoclonal -
Clone number
CPTC-APEX1-2 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Formalin-fixed, paraffin-embedded human Prostate Carcinoma tissue stained for APE1 using ab268072 at 2 μg/ml in immunohistochemical analysis.
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Immunofluorescence staining of human HeLa cells labeling APE1 with ab268072 followed by Goat anti-Mouse IgG-CF488 (Green). Phalloidin CF640 stains the membrane red.
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Formalin-fixed, paraffin-embedded human Prostate Carcinoma tissue stained for APE1 using ab268072 at 2 μg/ml in immunohistochemical analysis
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All lanes : Anti-APE1 antibody [CPTC-APEX1-2] (ab268072) at 2 µg/ml
Lane 1 : human A431 cell lysate
Lane 2 : human A549 cell lysate
Lane 3 : human PC3 cell lysate
Lane 4 : human HAP1 cell lysate
Lane 5 : human HepG2 cell lysate
Predicted band size: 35 kDa
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Flow Cytometryetric analysis of HeLa cells stained for APE1 using ab268072. Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
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Protein Array containing more than 19,000 full-length human proteins using ab268072. Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.