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Anti-APE1 antibody [CPTC-APEX1-2] (ab268072)

Anti-APE1 antibody [CPTC-APEX1-2] (ab268072)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [CPTC-APEX1-2] to APE1
  • Suitable for: Flow Cyt, IHC-P, Protein Array, WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-APE1 antibody [CPTC-APEX1-2]
    See all APE1 primary antibodies
  • Description

    Mouse monoclonal [CPTC-APEX1-2] to APE1
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant full length protein within Human APE1 aa 1-318. The exact sequence is proprietary.
    Database link: P27695

  • Positive control

    • WB: A431, A549, PC3, HAP1, HepG2 cell lysates. ICC/IF: HeLa cells. IHC: human Prostate Carcinoma. Flow Cyt: HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from Bioreactor concentrate
  • Clonality

    Monoclonal
  • Clone number

    CPTC-APEX1-2
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Base Excision Repair
    • Cardiovascular
    • Heart
    • Cardiogenesis
    • Transcription factors/regulators

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APE1 antibody [CPTC-APEX1-2] (ab268072)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APE1 antibody [CPTC-APEX1-2] (ab268072)

    Formalin-fixed, paraffin-embedded human Prostate Carcinoma tissue stained for APE1 using ab268072 at 2 μg/ml in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-APE1 antibody [CPTC-APEX1-2] (ab268072)
    Immunocytochemistry/ Immunofluorescence - Anti-APE1 antibody [CPTC-APEX1-2] (ab268072)

    Immunofluorescence staining of human HeLa cells labeling APE1 with ab268072 followed by Goat anti-Mouse IgG-CF488 (Green). Phalloidin CF640 stains the membrane red.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APE1 antibody [CPTC-APEX1-2] (ab268072)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APE1 antibody [CPTC-APEX1-2] (ab268072)

    Formalin-fixed, paraffin-embedded human Prostate Carcinoma tissue stained for APE1 using ab268072 at 2 μg/ml in immunohistochemical analysis

  • Western blot - Anti-APE1 antibody [CPTC-APEX1-2] (ab268072)
    Western blot - Anti-APE1 antibody [CPTC-APEX1-2] (ab268072)
    All lanes : Anti-APE1 antibody [CPTC-APEX1-2] (ab268072) at 2 µg/ml

    Lane 1 : human A431 cell lysate
    Lane 2 : human A549 cell lysate
    Lane 3 : human PC3 cell lysate
    Lane 4 : human HAP1 cell lysate
    Lane 5 : human HepG2 cell lysate

    Predicted band size: 35 kDa

  • Flow Cytometry - Anti-APE1 antibody [CPTC-APEX1-2] (ab268072)
    Flow Cytometry - Anti-APE1 antibody [CPTC-APEX1-2] (ab268072)

    Flow Cytometryetric analysis of HeLa cells stained for APE1 using ab268072. Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

  • Protein Array - Anti-APE1 antibody [CPTC-APEX1-2] (ab268072)
    Protein Array - Anti-APE1 antibody [CPTC-APEX1-2] (ab268072)

    Protein Array containing more than 19,000 full-length human proteins using ab268072. Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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