Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-EHMT2/G9A antibody [EPR4019(2)] (ab133482)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4019(2)] to EHMT2/G9A
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-EHMT2/G9A antibody [EPR4019(2)]
    See all EHMT2/G9A primary antibodies

  • Description

    Rabbit monoclonal [EPR4019(2)] to EHMT2/G9A

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human EHMT2/G9A aa 1150 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q96KQ7

  • Positive control

    • WB: HeLa, NCCIT, HepG2, and 293T cell lysates. IHC-P: Human gastric adenocarcinoma and lung squamous tissue.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-EHMT2/G9A antibody [EPR4019(2)] (ab133482)
    Western blot - Anti-EHMT2/G9A antibody [EPR4019(2)] (ab133482)
    All lanes : Anti-EHMT2/G9A antibody [EPR4019(2)] (ab133482) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : EHMT2/G9A knockout HeLa cell lysate
    Lane 3 : HEK-293 cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 132 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab133482 observed at 160 kDa. Red - loading control ab8245 observed at 37 kDa.

     ab133482 Anti-EHMT2/G9A antibody [EPR4019(2)] was shown to specifically react with EHMT2/G9A in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265149 (knockout cell lysate ab257080) was used. Wild-type and EHMT2/G9A knockout samples were subjected to SDS-PAGE. ab133482 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Western blot - Anti-EHMT2/G9A antibody [EPR4019(2)] (ab133482)
    Western blot - Anti-EHMT2/G9A antibody [EPR4019(2)] (ab133482)
    All lanes : Anti-EHMT2/G9A antibody [EPR4019(2)] (ab133482) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 cell lysate
    Lane 2 : EHMT2/G9A knockout HAP1 cell lysate
    Lane 3 : HEK293 cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 132 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab133482 observed at 170 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab133482 was shown to recognize EHMT2/G9A when EHMT2/G9A knockout samples were used, along with additional cross-reactive bands. Wild-type and EHMT2/G9A knockout samples were subjected to SDS-PAGE. ab133482 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-EHMT2/G9A antibody [EPR4019(2)] (ab133482)
    Western blot - Anti-EHMT2/G9A antibody [EPR4019(2)] (ab133482)
    All lanes : Anti-EHMT2/G9A antibody [EPR4019(2)] (ab133482) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : NCCIT cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat-anti-rabbit at 1/2000 dilution

    Predicted band size: 132 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHMT2/G9A antibody [EPR4019(2)] (ab133482)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHMT2/G9A antibody [EPR4019(2)] (ab133482)

    Immunohistochemical analysis of paraffin-embedded gastric adenocarcinoma tissue labelled with ab133482 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHMT2/G9A antibody [EPR4019(2)] (ab133482)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHMT2/G9A antibody [EPR4019(2)] (ab133482)

    Immunohistochemical analysis of paraffin-embedded lung squamous carcinoma tissue labelled with ab133482 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-EHMT2/G9A antibody [EPR4019(2)] (ab133482)
    Anti-EHMT2/G9A antibody [EPR4019(2)] (ab133482)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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