Anti-EHMT2/G9A antibody (ab40542)
Key features and details
- Rabbit polyclonal to EHMT2/G9A
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-EHMT2/G9A antibody
See all EHMT2/G9A primary antibodies -
Description
Rabbit polyclonal to EHMT2/G9A -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Fusion protein corresponding to Human EHMT2/ G9A aa 831-1001.
Database link: Q96KQ7
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-EHMT2/G9A antibody (ab40542)All lanes : Anti-EHMT2/G9A antibody (ab40542) at 1/2500 dilution
Lane 1 : Untransfected HeLa cell extract
Lane 2 : G9a transfected HeLa cell extract
Predicted band size: 132 kDa
Observed band size: 132 kDa
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHMT2/G9A antibody (ab40542)ab40542 (1:2000) staining EHMT2/ G9A in human prostate using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
