Anti-EHMT2/G9A antibody (ab183889)
Key features and details
- Rabbit polyclonal to EHMT2/G9A
- Suitable for: ICC/IF, IP, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-EHMT2/G9A antibody
See all EHMT2/G9A primary antibodies -
Description
Rabbit polyclonal to EHMT2/G9A -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig
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Immunogen
Synthetic peptide within Human EHMT2/ G9A aa 21-118. The exact sequence is proprietary.
Database link: Q96KQ7 -
Positive control
- 293T, A431, HeLa, HepG2 and A375 cell lysates. HeLa cells. HeLa xenograft tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-EHMT2/G9A antibody (ab183889)All lanes : Anti-EHMT2/G9A antibody (ab183889) at 1/1000 dilution
Lane 1 : 293T whole cell lysate/extract
Lane 2 : A431 whole cell lysate/extract
Lane 3 : HeLa whole cell lysate/extract
Lane 4 : HepG2 whole cell lysate/extract
Lane 5 : A375 whole cell lysate/extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 132 kDa
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Immunoprecipitation - Anti-EHMT2/G9A antibody (ab183889)Western blot analysis on immunoprecipitation. IP sample: 2 mg of HeLa whole cell lysate/extract. Lanes: 1) 40 μg of HeLa whole cell lysate/extract. 2) control with 2 μg of pre-immune rabbit IgG. 3) immunoprecipitation of KMT1C / G9a with 2 μg of ab183889. KMT1C / G9a immunoprecipitate was detected by ab183889 at a 1/1000 dilution.
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Immunocytochemistry/ Immunofluorescence - Anti-EHMT2/G9A antibody (ab183889)Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde for 5 minutes, labeling EHMT2/ G9A using ab183889 at a 1/500 dilution (green). Blue: Hoechst 33342 staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHMT2/G9A antibody (ab183889)Immunohistochemical analysis of paraffin-embedded HeLa xenograft tissue, labeling EHMT2/ G9A using ab183889 at a 1/500 dilution.
