violetFluor™ 450 Anti-Ly6g antibody [RB6-8C5] (ab253070)
![violetFluor™ 450 Anti-Ly6g antibody [RB6-8C5] (ab253070)](https://www.abcam.com/ps/products/253/ab253070/Images/ab253070-343620-ab253070343620antily6gratmonoclonalantibodyrb68c5violetfluor450flowcytometrymouse.jpg "violetFluor™ 450 Anti-Ly6g antibody [RB6-8C5] (ab253070)")
Key features and details
- violetFluor™ 450 Rat monoclonal [RB6-8C5] to Ly6g
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: violetFluor™ 450. Ex: 405nm, Em: 450nm
- Isotype: IgG2b
Overview
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Product name
violetFluor™ 450 Anti-Ly6g antibody [RB6-8C5]
See all Ly6g primary antibodies -
Description
violetFluor™ 450 Rat monoclonal [RB6-8C5] to Ly6g -
Host species
Rat -
Conjugation
violetFluor™ 450. Ex: 405nm, Em: 450nm -
Specificity
In the mouse, the RB6-8C5 antibody is typically used in combination with the macrophage labeling antibody M1/70 (Anti-CD11b) for phenotypic analysis of monocytes, macrophages and granulocytes. Note: The RB6-8C5 antibody has been reported to cross-react with Ly-6C on cells expressing this antigen (Fleming et al. 1993. J. Immunol. 151:2399-2408 and Sasmono et al. 2007. J. Leukoc. Biol. 82: 111-123) and has been cited in the literature for identification of Ly-6G/Ly-6C. Other reports suggest that this antibody is specific for Ly-6G, without cross-reactivity for Ly-6C (Nagendra S. and Schlueter AJ. 2003. Cytometry A, 58(2): 195-200.
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Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Mouse
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Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- Flow cyt: Mouse C57Bl/6 bone marrow cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.16% Sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from tissue culture supernatant. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. -
Clonality
Monoclonal -
Clone number
RB6-8C5 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Images
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Flow Cytometry - violetFluor™ 450 Anti-Ly6g antibody [RB6-8C5] (ab253070)
Flow cytometric analysis of mouse C57Bl/6 bone marrow cells stained for Ly6g using ab253070 at 0.06 μg per reaction (blue) or a rat IgG2b violetFluor™ 450 isotype control (black dashed line).
