Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-INPP4B antibody [EPR3108Y] - BSA and Azide free (ab247515)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3108Y] to INPP4B - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-INPP4B antibody [EPR3108Y] - BSA and Azide free
    See all INPP4B primary antibodies

  • Description

    Rabbit monoclonal [EPR3108Y] to INPP4B - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, Flow Cyt, WBmore details
    Unsuitable for: ICC/IF or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt: MCF7 cells

  • General notes

    Ab247515 is the carrier-free version of ab81269. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247515 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-INPP4B antibody [EPR3108Y] - BSA and Azide free (ab247515)
    Western blot - Anti-INPP4B antibody [EPR3108Y] - BSA and Azide free (ab247515)
    All lanes : Anti-INPP4B antibody [EPR3108Y] (ab81269) at 1/5000 dilution

    Lane 1 : MCF7 cell lysate
    Lane 2 : Fetal skeletal muscle tissue lysate
    Lane 3 : Fetal heart tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 107 kDa
    Observed band size: 107 kDa



    This data was developed using ab81269, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-INPP4B antibody [EPR3108Y] - BSA and Azide free (ab247515)
    Flow Cytometry - Anti-INPP4B antibody [EPR3108Y] - BSA and Azide free (ab247515)
    This data was developed using ab81269, the same antibody clone in a different buffer formulation.
    Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling INPP4B with purified ab81269 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INPP4B antibody [EPR3108Y] - BSA and Azide free (ab247515)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INPP4B antibody [EPR3108Y] - BSA and Azide free (ab247515)
    This data was developed using ab81269, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded muscle tissue using ab81269 at 1/50 dilution.
  • Anti-INPP4B antibody [EPR3108Y] - BSA and Azide free (ab247515)
    Anti-INPP4B antibody [EPR3108Y] - BSA and Azide free (ab247515)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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