Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-INPP4B antibody [EPR3108Y] (ab81269)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3108Y] to INPP4B
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-INPP4B antibody [EPR3108Y]
    See all INPP4B primary antibodies

  • Description

    Rabbit monoclonal [EPR3108Y] to INPP4B

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human INPP4B aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: O15327

  • Positive control

    • MCF7, fetal skeletal muscle, fetal heart lysates and muscle tissue. Flow Cyt: MCF7 cells

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR3108Y

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INPP4B antibody [EPR3108Y] (ab81269)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INPP4B antibody [EPR3108Y] (ab81269) Image from Stjernström A et al., Cancer Medicine. 2014;3(2):337-348. Fig 1(A).; doi: 10.1002/cam4.191. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.

    Immunohistochemistry analysis of formalin-fixed and paraffin-embedded human squamous cell carcinoma tissue sectioned at 4 μm and placed at 60°C for 1 hour. Slides were deparaffinized and antigen retrieval was carried out by microwaving the slides in Tris-EDTA buffer pH 9 at 650 W for 30 minutes. Samples were incubated with ab81269 (1/50) for 25 minutes at room temperature.

    INPP4B positive expression shows strong cytoplasmic staining. Magnification: X40.

  • Western blot - Anti-INPP4B antibody [EPR3108Y] (ab81269)
    Western blot - Anti-INPP4B antibody [EPR3108Y] (ab81269)
    All lanes : Anti-INPP4B antibody [EPR3108Y] (ab81269) at 1/5000 dilution

    Lane 1 : MCF7 cell lysate
    Lane 2 : Fetal skeletal muscle lysate
    Lane 3 : Fetal heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 107 kDa
    Observed band size: 107 kDa

  • Flow Cytometry - Anti-INPP4B antibody [EPR3108Y] (ab81269)
    Flow Cytometry - Anti-INPP4B antibody [EPR3108Y] (ab81269)
    Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling INPP4B with purified ab81269 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INPP4B antibody [EPR3108Y] (ab81269)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INPP4B antibody [EPR3108Y] (ab81269) Image from Stjernström A et al., Cancer Medicine. 2014;3(2):337-348. Fig 1(B).; doi: 10.1002/cam4.191. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.

    Immunohistochemistry analysis of formalin-fixed and paraffin-embedded human squamous cell carcinoma tissue samples sectioned at 4 μm and placed at 60°C for 1 hour. Slides were deparaffinized and antigen retrieval was carried out by microwaving the slides in Tris-EDTA buffer pH 9 at 650 W for 30 minutes. Samples were incubated with ab81269 (1/50) for 25 minutes at room temperature.

    INPP4B negative expression. Magnification: X40.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INPP4B antibody [EPR3108Y] (ab81269)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INPP4B antibody [EPR3108Y] (ab81269) Image from Stjernström A et al., Cancer Medicine. 2014;3(2):337-348. Fig 1(C).; doi: 10.1002/cam4.191. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.

    Immunohistochemistry analysis of formalin-fixed and paraffin-embedded human skeletal muscle tissue (negative control) sectioned at 4 μm and placed at 60°C for 1 hour. Slides were deparaffinized and antigen retrieval was carried out by microwaving the slides in Tris-EDTA buffer pH 9 at 650 W for 30 minutes. Samples were incubated with primary antibody substituted for antibody diluent for 25 minutes at room temperature.

    Magnification: X40.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INPP4B antibody [EPR3108Y] (ab81269)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INPP4B antibody [EPR3108Y] (ab81269)
    Immunohistochemical analysis of paraffin-embedded muscle tissue using ab81269 at 1/50 dilution.
  • Anti-INPP4B antibody [EPR3108Y] (ab81269)
    Anti-INPP4B antibody [EPR3108Y] (ab81269)

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