Anti-Monoamine Oxidase B/MAOB antibody [EPR24131-79] (ab259928)
![Anti-Monoamine Oxidase B/MAOB antibody [EPR24131-79] (ab259928)](https://www.abcam.com/ps/products/259/ab259928/Images/ab259928-451592-anti-monoamine-oxidase-b-antibody-epr2413179-western-blot-lysate.jpg "Anti-Monoamine Oxidase B/MAOB antibody [EPR24131-79] (ab259928)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24131-79] to Monoamine Oxidase B/MAOB
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-Monoamine Oxidase B/MAOB antibody [EPR24131-79]
See all Monoamine Oxidase B/MAOB primary antibodies -
Description
Rabbit monoclonal [EPR24131-79] to Monoamine Oxidase B/MAOB -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseRatIP MouseWB MouseRat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse brain and liver tissue lysates; Rat brain and liver tissue lysate; E.coli extracts containing his-tagged mouse Amine oxidase [flavin-containing] B recombinant protein (aa 2-489). IHC-P: Mouse liver; Rat liver. IP: Mouse liver tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24131-79 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Monoamine Oxidase B/MAOB antibody (ab259928)All lanes : Anti-Monoamine Oxidase B/MAOB antibody [EPR24131-79] (ab259928) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse liver tissue lysate
Lane 3 : Mouse testis tissue lysate
Lane 4 : Rat brain tissue lysate
Lane 5 : Rat liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 59 kDa
Observed band size: 59 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
The expression profile observed is consistent with what has been described in the literature (PMID: 8058968).
Low expression: mouse testis.
Protein aggregates may be caused by boiling of samples .
Exposure time: 15 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab259928)Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Monoamine Oxidase B with ab259928 at 1/1000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasm staining on mouse liver. The section was incubated with ab259928 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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Western blot - Anti-Monoamine Oxidase B/MAOB antibody (ab259928)All lanes : Anti-Monoamine Oxidase B/MAOB antibody [EPR24131-79] (ab259928) at 1/1000 dilution
Lane 1 : E.coli extracts containing his-tagged mouse Amine oxidase flavin-containi B recombinant protein (aa 2-489) at 10 µl
Lane 2 : E.coli extracts containing his-tagged mouse Amine oxidase flavin-containi A recombinant protein (aa 1-497) at 4 µl
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 59 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
Samples are non-boiled as boiling may cause protein aggregates.
Exposure time: 15 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab259928)Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Monoamine Oxidase B with ab259928 at 1/1000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasm staining on rat liver. The section was incubated with ab259928 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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Immunoprecipitation - (ab259928)Monoamine Oxidase B was immunoprecipitated from 0.35 mg Mouse liver tissue lysate 10 ug with ab259928 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259928 at 1/2000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse liver tissue lysate 10 ug
Lane 2: ab259928 IP in Mouse liver tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab259928 in mouse liver tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 32 seconds
Sample loaded onto lane 1 are non-boiled as boiling may cause protein aggregates.
