Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SIRP alpha antibody [EPR22930-163] (ab260039)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22930-163] to SIRP alpha
  • Suitable for: ICC/IF, IHC-P, WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-SIRP alpha antibody [EPR22930-163]
    See all SIRP alpha primary antibodies

  • Description

    Rabbit monoclonal [EPR22930-163] to SIRP alpha

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human brain, U-937, SK-MEL-28 and SK-MEL-2 lysates. IHC-P: Human cerebrum and spleen tissues. ICC/IF: SK-MEL-28 cells. Flow Cyt: THP-1 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRP alpha antibody [EPR22930-163] (ab260039)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRP alpha antibody [EPR22930-163] (ab260039)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling SIRP alpha with ab260039 at 1/500 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the human spleen is observed. The section was incubated with ab260039 for 15 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Western blot - Anti-SIRP alpha antibody [EPR22930-163] (ab260039)
    Western blot - Anti-SIRP alpha antibody [EPR22930-163] (ab260039)
    All lanes : Anti-SIRP alpha antibody [EPR22930-163] (ab260039) at 1/1000 dilution

    Lane 1 : Human brain lysate
    Lane 2 : U-937 (human histiocytic lymphoma monocyte) whole cell lysate
    Lane 3 : SK-MEL-28 (human malignant melanoma) whole cell lysate
    Lane 4 : SK-MEL-2 (human skin malignant melanoma) whole cell lysate
    Lane 5 : Jurkat (human T-cell leukemia lymphocyte) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 55 kDa
    Observed band size: 85 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    There are many forms of SIRP alpha because of different glycosylation and phosphotyrosine modification.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 9712903). 

    Negative control: Jurkat (PMID: 17982459). 

    Exposure time: 3 minutes

  • Flow Cytometry - Anti-SIRP alpha antibody [EPR22930-163] (ab260039)
    Flow Cytometry - Anti-SIRP alpha antibody [EPR22930-163] (ab260039)

    Flow cytometric analysis of Jurkat (Human T cell leukemia T lymphocyte, Left) / THP-1 (Human monocytic leukemia monocyte, Right) cells labeling SIRP alpha with ab260039 at 1\600 compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.

    Negative control: Jurkat.

    Gated on viable cells.

  • Immunocytochemistry/ Immunofluorescence - Anti-SIRP alpha antibody [EPR22930-163] (ab260039)
    Immunocytochemistry/ Immunofluorescence - Anti-SIRP alpha antibody [EPR22930-163] (ab260039)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SK-MEL-28 (human malignant melanoma melanoma) cells labeling SIRP alpha with ab260039 at 1/250 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in SK-MEL-28 cells Negative control: Jurkat (PMID: 17982459) is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRP alpha antibody [EPR22930-163] (ab260039)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRP alpha antibody [EPR22930-163] (ab260039)

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling SIRP alpha with ab260039 at 1/500 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the human cerebrum (PMID: 9062191) is observed. The section was incubated with ab260039 for 15 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Anti-SIRP alpha antibody [EPR22930-163] (ab260039)
    Anti-SIRP alpha antibody [EPR22930-163] (ab260039)

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