Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸ 10 µl 110 563 ₸

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Anti-SIRP alpha antibody [EPR22930-163] (ab260039)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22930-163] to SIRP alpha
Suitable for: ICC/IF, IHC-P, WB, Flow Cyt
Reacts with: Human

Product name

Anti-SIRP alpha antibody [EPR22930-163]
See all SIRP alpha primary antibodies
Description

Rabbit monoclonal [EPR22930-163] to SIRP alpha
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human brain, U-937, SK-MEL-28 and SK-MEL-2 lysates. IHC-P: Human cerebrum and spleen tissues. ICC/IF: SK-MEL-28 cells. Flow Cyt: THP-1 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22930-163
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRP alpha antibody [EPR22930-163] (ab260039)

Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling SIRP alpha with ab260039 at 1/500 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the human spleen is observed. The section was incubated with ab260039 for 15 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control: Ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Western blot - Anti-SIRP alpha antibody [EPR22930-163] (ab260039)

All lanes : Anti-SIRP alpha antibody [EPR22930-163] (ab260039) at 1/1000 dilution

Lane 1 : Human brain lysate
Lane 2 : U-937 (human histiocytic lymphoma monocyte) whole cell lysate
Lane 3 : SK-MEL-28 (human malignant melanoma) whole cell lysate
Lane 4 : SK-MEL-2 (human skin malignant melanoma) whole cell lysate
Lane 5 : Jurkat (human T-cell leukemia lymphocyte) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 55 kDa
Observed band size: 85 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

There are many forms of SIRP alpha because of different glycosylation and phosphotyrosine modification.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 9712903).

Negative control: Jurkat (PMID: 17982459).

Exposure time: 3 minutes
Flow Cytometry - Anti-SIRP alpha antibody [EPR22930-163] (ab260039)

Flow cytometric analysis of Jurkat (Human T cell leukemia T lymphocyte, Left) / THP-1 (Human monocytic leukemia monocyte, Right) cells labeling SIRP alpha with ab260039 at 1\600 compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 was used as the secondary antibody.

Negative control: Jurkat.

Gated on viable cells.
Immunocytochemistry/ Immunofluorescence - Anti-SIRP alpha antibody [EPR22930-163] (ab260039)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SK-MEL-28 (human malignant melanoma melanoma) cells labeling SIRP alpha with ab260039 at 1/250 dilution, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in SK-MEL-28 cells Negative control: Jurkat (PMID: 17982459) is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control: ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRP alpha antibody [EPR22930-163] (ab260039)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling SIRP alpha with ab260039 at 1/500 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the human cerebrum (PMID: 9062191) is observed. The section was incubated with ab260039 for 15 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control: Ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Anti-SIRP alpha antibody [EPR22930-163] (ab260039)

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