Antibodies, Proteins, Kits and Reagents for Life Science

Anti-ERK1 + ERK2 (phospho T202 + Y204) antibody [ERK12T202Y204-A11] (ab278538)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [ERK12T202Y204-A11] to ERK1 + ERK2 (phospho T202 + Y204)
Suitable for: WB, Flow Cyt
Reacts with: Mouse, Human

Product name

Anti-ERK1 + ERK2 (phospho T202 + Y204) antibody [ERK12T202Y204-A11]
See all ERK1 + ERK2 primary antibodies
Description

Rabbit monoclonal [ERK12T202Y204-A11] to ERK1 + ERK2 (phospho T202 + Y204)
Host species

Rabbit
Tested Applications & Species

Application Species
Flow Cyt
Mouse

Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human ERK1 + ERK2 (phospho T202 + Y204). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. Uniprot P27361/ P28482
Database link: P27361

Run BLAST with Run BLAST with
Positive control
- WB: Ramos (treated with PMA or TPA) cell extract; NIH/3T3 (treated with TPA) cell extract. Flow cyt: L929 cells; Jurkat cells (treated with PMA).
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Application	Species
Flow Cyt	Mouse Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.02% Sodium azide
Constituents: 49.88% PBS, 0.1% BSA, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A/G purified
Clonality

Monoclonal
Clone number

ERK12T202Y204-A11
Isotype

IgG
Light chain type

kappa
Research areas
- Cancer
- Cell Death
- Apoptosis
- Other

Flow Cytometry - Anti-ERK1 + ERK2 (phospho T202 + Y204) antibody [ERK12T202Y204-A11] (ab278538)

ab278538 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of L929 cells secondary antibody only (blue) or 0.1 µg/mL of isotype control (orange) or of ab278538.
Western blot - Anti-ERK1 + ERK2 (phospho T202 + Y204) antibody [ERK12T202Y204-A11] (ab278538)

All lanes : Anti-ERK1 + ERK2 (phospho T202 + Y204) antibody [ERK12T202Y204-A11] (ab278538) at 0.05 µg/ml

Lane 1 : Ramos (treated with U0126) cell extract
Lane 2 : Ramos (treated with TPA) cell extract

Predicted band size: 41 kDa

Western blot analysis of Ramos cells treated with U0126 or treated with TPA using 0.05 μg/mL of ab278538.
Flow Cytometry - Anti-ERK1 + ERK2 (phospho T202 + Y204) antibody [ERK12T202Y204-A11] (ab278538)

Flow cytometric analysis of Jurkat cells, secondary antibody only negative control (blue), or treated with U0126 (red) or with PMA (green) using ab278538.
Western blot - Anti-ERK1 + ERK2 (phospho T202 + Y204) antibody [ERK12T202Y204-A11] (ab278538)

All lanes : Anti-ERK1 + ERK2 (phospho T202 + Y204) antibody [ERK12T202Y204-A11] (ab278538) at 0.1 µg/ml

Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen)(treated with TPA) cell extract .
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) (treated with U0126) cell extract.

Predicted band size: 41 kDa
Western blot - Anti-ERK1 + ERK2 (phospho T202 + Y204) antibody [ERK12T202Y204-A11] (ab278538)

All lanes : Anti-ERK1 + ERK2 (phospho T202 + Y204) antibody [ERK12T202Y204-A11] (ab278538) at 0.1 µg/ml

Lane 1 : Ramos cell extract untreated
Lane 2 : Ramos cell extract treated with PMA
Lane 3 : Ramos cell extract treated with U0126
Lane 4 : Ramos cell extract treated with U0126 followed by treatment with PMA

Predicted band size: 41 kDa
Anti-ERK1 + ERK2 (phospho T202 + Y204) antibody [ERK12T202Y204-A11] (ab278538)

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