Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ERK1 + ERK2 (phospho T202 + Y204) antibody [ERK12T202Y204-A11] (ab278538)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [ERK12T202Y204-A11] to ERK1 + ERK2 (phospho T202 + Y204)
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-ERK1 + ERK2 (phospho T202 + Y204) antibody [ERK12T202Y204-A11]
    See all ERK1 + ERK2 primary antibodies

  • Description

    Rabbit monoclonal [ERK12T202Y204-A11] to ERK1 + ERK2 (phospho T202 + Y204)

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human ERK1 + ERK2 (phospho T202 + Y204). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. Uniprot P27361/ P28482
    Database link: P27361

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Ramos (treated with PMA or TPA) cell extract; NIH/3T3 (treated with TPA) cell extract. Flow cyt: L929 cells; Jurkat cells (treated with PMA).

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Flow Cytometry - Anti-ERK1 + ERK2 (phospho T202 + Y204) antibody [ERK12T202Y204-A11] (ab278538)
    Flow Cytometry - Anti-ERK1 + ERK2 (phospho T202 + Y204) antibody [ERK12T202Y204-A11] (ab278538)
    ab278538 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of L929 cells secondary antibody only (blue) or 0.1 µg/mL of isotype control (orange) or of ab278538.
  • Western blot - Anti-ERK1 + ERK2 (phospho T202 + Y204) antibody [ERK12T202Y204-A11] (ab278538)
    Western blot - Anti-ERK1 + ERK2 (phospho T202 + Y204) antibody [ERK12T202Y204-A11] (ab278538)
    All lanes : Anti-ERK1 + ERK2 (phospho T202 + Y204) antibody [ERK12T202Y204-A11] (ab278538) at 0.05 µg/ml

    Lane 1 : Ramos (treated with U0126) cell extract
    Lane 2 : Ramos (treated with TPA) cell extract

    Predicted band size: 41 kDa



    Western blot analysis of Ramos cells treated with U0126 or treated with TPA using 0.05 μg/mL of ab278538.

  • Flow Cytometry - Anti-ERK1 + ERK2 (phospho T202 + Y204) antibody [ERK12T202Y204-A11] (ab278538)
    Flow Cytometry - Anti-ERK1 + ERK2 (phospho T202 + Y204) antibody [ERK12T202Y204-A11] (ab278538)

    Flow cytometric analysis of Jurkat cells, secondary antibody only negative control (blue), or treated with U0126 (red) or with PMA (green) using ab278538.

  • Western blot - Anti-ERK1 + ERK2 (phospho T202 + Y204) antibody [ERK12T202Y204-A11] (ab278538)
    Western blot - Anti-ERK1 + ERK2 (phospho T202 + Y204) antibody [ERK12T202Y204-A11] (ab278538)
    All lanes : Anti-ERK1 + ERK2 (phospho T202 + Y204) antibody [ERK12T202Y204-A11] (ab278538) at 0.1 µg/ml

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen)(treated with TPA) cell extract .
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) (treated with U0126) cell extract.

    Predicted band size: 41 kDa

  • Western blot - Anti-ERK1 + ERK2 (phospho T202 + Y204) antibody [ERK12T202Y204-A11] (ab278538)
    Western blot - Anti-ERK1 + ERK2 (phospho T202 + Y204) antibody [ERK12T202Y204-A11] (ab278538)
    All lanes : Anti-ERK1 + ERK2 (phospho T202 + Y204) antibody [ERK12T202Y204-A11] (ab278538) at 0.1 µg/ml

    Lane 1 : Ramos cell extract untreated
    Lane 2 : Ramos cell extract treated with PMA
    Lane 3 : Ramos cell extract treated with U0126
    Lane 4 : Ramos cell extract treated with U0126 followed by treatment with PMA

    Predicted band size: 41 kDa

  • Anti-ERK1 + ERK2 (phospho T202 + Y204) antibody [ERK12T202Y204-A11] (ab278538)
    Anti-ERK1 + ERK2 (phospho T202 + Y204) antibody [ERK12T202Y204-A11] (ab278538)

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