Plasmin Activity Assay Kit (Colorimetric) (ab273301)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate
- Sample type: Plasma
Overview
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Product name
Plasmin Activity Assay Kit (Colorimetric)
See all Plasmin kits -
Detection method
Colorimetric -
Sample type
Plasma -
Assay type
Quantitative -
Assay duration
Multiple steps standard assay -
Product overview
Plasmin Activity Assay Kit (Colorimetric) (ab273301) utilizes the ability of active plasmin to hydrolyze the synthetic substrate thereby releasing pNA (chromophore), which can be easily quantified at OD 405 nm. The stable colorimetric signal is directly proportional to the plasmin activity in samples. The kit includes a specific inhibitor that can be used to compensate for the potential non-specific background in unknown samples. Our assay kit is simple, specific and can detect as low as 40 µU of plasmin activity in samples.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Tested applications
Suitable for: Othermore details -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests pNA Standard 1 x 20µl Plasmin Assay Buffer 1 x 50ml Plasmin Inhibitor Mix (Lyophilized) 1 vial Plasmin Positive Control 1 x 15µl Plasmin Substrate 1 x 400µl -
Research areas
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Function
Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells.
Angiostatin is an angiogenesis inhibitor that blocks neovascularization and growth of experimental primary and metastatic tumors in vivo. -
Tissue specificity
Present in plasma and many other extracellular fluids. It is synthesized in the liver. -
Involvement in disease
Plasminogen deficiency -
Sequence similarities
Belongs to the peptidase S1 family. Plasminogen subfamily.
Contains 5 kringle domains.
Contains 1 PAN domain.
Contains 1 peptidase S1 domain. -
Domain
Kringle domains mediate interaction with CSPG4. -
Post-translational
modificationsN-linked glycan contains N-acetyllactosamine and sialic acid. O-linked glycans consist of Gal-GalNAc disaccharide modified with up to 2 sialic acid residues (microheterogeneity).
In the presence of the inhibitor, the activation involves only cleavage after Arg-580, yielding two chains held together by two disulfide bonds. In the absence of the inhibitor, the activation involves additionally the removal of the activation peptide. -
Cellular localization
Secreted. Locates to the cell surface where it is proteolytically cleaved to produce the active plasmin. Interaction with HRG tethers it to the cell surface. - Information by UniProt
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Alternative names
- Plasmin light chain B
- Plasminogen
- PLG
- PLMN_HUMAN
Images
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Example data
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
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Example dataMeasurement of purified Human Plasmin with or without the Plasmin Inhibitor Mix.
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Example dataMeasurement of Plasmin activity in Human Pooled EDTA-treated Plasma Sample (50 µl, 1:20 dilution) in the presence and absence of Activating Buffer containing Urokinase.