Human Antithrombin III ELISA Kit (ab220073)
Key features and details
- Sensitivity: 0.03 µg/ml
- Range: 0.063 µg/ml - 4 µg/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
Overview
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Product name
Human Antithrombin III ELISA Kit
See all Antithrombin III/ATIII kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 20 3.1% 2 20 3.2% 3 20 3.6% Inter-assay Sample n Mean SD CV% 1 20 8.9% 2 20 9% 3 20 9.1% -
Sample type
Serum, Plasma -
Assay type
Competitive -
Sensitivity
= 0.03 µg/ml -
Range
0.063 µg/ml - 4 µg/ml -
Recovery
96.5 %
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Assay time
2h 40m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
The Human Antithrombin III ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab220073) is designed for detection of Human Antithrombin III (AT3) in plasma and serum samples.
This assay employs a quantitative competitive enzyme immunoassay technique that measures Antithrombin III in less than 3 hours. A polyclonal antibody specific for Antithrombin III has been pre-coated onto a 96-well microplate with removable strips. Antithrombin III in standards and samples compete with a biotinylated Antithrombin III sandwiched by the immobilized antibody and streptavidin-peroxidase conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Notes
The serine protease inhibitor antithrombin III, the most important natural inhibitor of thrombin activity, has been shown to exert marked anti-inflammatory properties. AT3 levels are positively correlated with plasma total cholesterol levels, plasma low-density lipoprotein cholesterol levels, plasma triglycerides and D-dimer levels.
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Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 30ml 20X Wash Buffer Concentrate 1 x 30ml 4X Biotinylated Human AT3 protein (Lyophilized) 1 vial Anti-Human AT3 coated Microplate (12 x 8 wells) 1 unit Antithrombin III Standard 1 vial Chromogen Substrate 1 x 8ml Sealing Tapes 3 units Stop Solution 1 x 12ml -
Research areas
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Function
Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin. -
Tissue specificity
Found in plasma. -
Involvement in disease
Defects in SERPINC1 are the cause of antithrombin III deficiency (AT3D) [MIM:613118]. AT3D is an important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. AT3D is classified into 4 types. Type I: characterized by a 50% decrease in antigenic and functional levels. Type II: has defects affecting the thrombin-binding domain. Type III: alteration of the heparin-binding domain. Plasma AT-III antigen levels are normal in type II and III. Type IV: consists of miscellaneous group of unclassifiable mutations. -
Sequence similarities
Belongs to the serpin family. -
Post-translational
modificationsPhosphorylation sites are present in the extracelllular medium. -
Cellular localization
Secreted > extracellular space. - Information by UniProt
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Alternative names
- ANT3_HUMAN
- Antithrombin
- Antithrombin III
see all -
Database links
- Entrez Gene: 462 Human
- Omim: 107300 Human
- SwissProt: P01008 Human
- Unigene: 75599 Human
Images
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Human ATIII Standard curve (ab220073)
Human ATIII Standard curve (ab220073)
