Human Tissue Type Plasminogen Activator ELISA Kit (TPA) (ab108914)
Key features and details
- Sensitivity: 0.03 ng/ml
- Range: 0.031 ng/ml - 2 ng/ml
- Sample type: Cell culture supernatant, Milk, Plasma, Saliva, Serum, Tissue Extracts, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Tissue Type Plasminogen Activator ELISA Kit (TPA)
See all Tissue Plasminogen Activator kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 4.8% Inter-assay Sample n Mean SD CV% Overall 9.9% -
Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
= 0.03 ng/ml -
Range
0.031 ng/ml - 2 ng/ml -
Recovery
98 %
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Assay time
4h 0m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Tissue Type Plasminogen Activator (TPA) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Tissue Type Plasminogen Activator in plasma, serum, urine, saliva, milk, cell culture supernatants and tissue extracts.
A TPA specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a TPA specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of TPA captured in plate.
Get results in 90 minutes with Human Tissue Plasminogen Activator ELISA Kit (ab190812) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30µl 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human TPA Antibody 1 x 120µl Chromogen Substrate 1 x 8ml Sealing Tapes 3 units Stop Solution 1 x 12ml TPA Microplate (12 x 8 well strips) 1 unit TPA Standard 1 vial -
Research areas
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Function
Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Play a direct role in facilitating neuronal migration. -
Tissue specificity
Synthesized in numerous tissues (including tumors) and secreted into most extracellular body fluids, such as plasma, uterine fluid, saliva, gingival crevicular fluid, tears, seminal fluid, and milk. -
Involvement in disease
Note=Increased activity of TPA results in increased fibrinolysis of fibrin blood clots that is associated with excessive bleeding. Defective release of TPA results in hypofibrinolysis that can lead to thrombosis or embolism. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 EGF-like domain.
Contains 1 fibronectin type-I domain.
Contains 2 kringle domains.
Contains 1 peptidase S1 domain. -
Domain
Both FN1 and one of the kringle domains are required for binding to fibrin.
Both FN1 and EGF-like domains are important for binding to LRP1.
The FN1 domain mediates binding to annexin A2.
The second kringle domain is implicated in binding to cytokeratin-8 and to the endothelial cell surface binding site. -
Post-translational
modificationsThe single chain, almost fully active enzyme, can be further processed into a two-chain fully active form by a cleavage after Arg-310 catalyzed by plasmin, tissue kallikrein or factor Xa.
Differential cell-specific N-linked glycosylation gives rise to two glycoforms, type I (glycosylated at Asn-219) and type II (not glycosylated at Asn-219). The single chain type I glycoform is less readily converted into the two-chain form by plasmin, and the two-chain type I glycoform has a lower activity than the two-chain type II glycoform in the presence of fibrin.
N-glycosylation of Asn-152; the bound oligomannosidic glycan is involved in the interaction with the mannose receptor.
Characterization of O-linked glycan was studied in Bowes melanoma cell line. -
Cellular localization
Secreted > extracellular space. - Information by UniProt
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Alternative names
- Alteplase
- DKFZp686I03148
- Plasminogen activator tissue
see all -
Database links
- Entrez Gene: 5327 Human
- Omim: 173370 Human
- SwissProt: P00750 Human
- Unigene: 491582 Human
Images
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TPA - Human ELISA Kit (ab108914)
Tissue Type Plasminogen Activator measured in biological fluids showing quantity (ng) per mL of tested sample. Samples diluted 2-10 fold.
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TPA - Human ELISA Kit (ab108914)Tissue Type Plasminogen Activator measured in biological fluids showing quantity (ng) per mL of tested sample. Human samples diluted 3-27 fold. Rat and mouse samples diluted 1-10 fold.
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Typical Standard CurveRepresentative Standard Curve Using ab108914.
