Anti-AKT (phospho T308) antibody (ab38449)
Key features and details
- Rabbit polyclonal to AKT (phospho T308)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-AKT (phospho T308) antibody
See all AKT primary antibodies -
Description
Rabbit polyclonal to AKT (phospho T308) -
Host species
Rabbit -
Specificity
This antibody was made against a peptide directed against the phosphorylated form of AKT1 at T308, but due to a high degree of homology it is predicted to cross react with AKT2 and AKT3 if they are phosphorylated at the corresponding residue.
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Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human AKT aa 250-350 (phospho T308).
Database link: P31749 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-AKT (phospho T308) antibody (ab38449)All lanes : Anti-AKT (phospho T308) antibody (ab38449) at 1/1000 dilution
Lane 1 : HeLa cells
Lane 2 : HeLa cells treated with 0.01 U/mL Insulin for 15 minutes
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (H+L) HRP at 1/10000 dilution
Predicted band size: 56 kDa10% gel.
Running conditions: 60v, 30min; 120v 60min
Transfer conditions: 150mA 120min Nitrocellulose membrane.
Blocking conditions: 5% non-fat milk in TBST, RT, 90min.
Primary antibody incubation: 4?, overnight
Secondary antibody incubation: room temperature for 45 minutes
Washing condition: 5 ml TBST, 4 x 5min
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT (phospho T308) antibody (ab38449)Immunohistochemical analysis of AKT (phospho T308) expression in paraffin embedded human lung carcinoma tissue, using ab38449 (1/50). Right-hand panel represents a negative control where ab38449 was pre-incubated with the immunizing (blocking) peptide. -
Western blot - Anti-AKT (phospho T308) antibody (ab38449)All lanes : Anti-AKT (phospho T308) antibody (ab38449) at 1/500 dilution
Lane 1 : 293 cell lysate - untreated
Lane 2 : 293 cell lysate - treated with insulin
Lysates/proteins at 30 µg per lane.
Predicted band size: 56 kDa
Observed band size: 56 kDa
