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Anti-Smac/Diablo antibody [8H5AA3] (ab110288)

Key features and details

  • Mouse monoclonal [8H5AA3] to Smac/Diablo
  • Suitable for: ICC, Flow Cyt, IHC-P
  • Reacts with: Human
  • Isotype: IgG2a

Overview

  • Product name

    Anti-Smac/Diablo antibody [8H5AA3]
    See all Smac/Diablo primary antibodies

  • Description

    Mouse monoclonal [8H5AA3] to Smac/Diablo

  • Host species

    Mouse

  • Tested applications

    Suitable for: ICC, Flow Cyt, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein. This information is considered to be commercially sensitive.

  • Positive control

    • Human HDFn cells and HeLa cells. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal testis.

  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Images

  • Flow Cytometry - Anti-Smac / Diablo antibody [8H5AA3] (ab110288)
    Flow Cytometry - Anti-Smac / Diablo antibody [8H5AA3] (ab110288)
    HeLa cells were stained with 1 µg/mL Smac / Diablo antibody (ab110288) (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
  • Immunocytochemistry - Anti-Smac / Diablo antibody [8H5AA3] (ab110288)
    Immunocytochemistry - Anti-Smac / Diablo antibody [8H5AA3] (ab110288)
    Immunocytochemistry image of Smac / Diablo stained human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with the antibody (ab110288) at 1µg/ml for 2h at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smac / Diablo antibody [8H5AA3] (ab110288)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smac / Diablo antibody [8H5AA3] (ab110288)

    IHC image of Smac / Diablo staining in Human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab110288, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

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