Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Smac/Diablo antibody (ab66290)

Key features and details

  • Rabbit polyclonal to Smac/Diablo
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Smac/Diablo antibody
    See all Smac/Diablo primary antibodies

  • Description

    Rabbit polyclonal to Smac/Diablo

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human Smac/ Diablo aa 50-150 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab66289)

  • Positive control

    • This antibody gave a positive signal in the following Human Whole Cell Lysates: HeLa, Jurkat, A431, HEK293, HepG2, MCF7, SHSY-5Y and U2OS

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab66290 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
All applications
Mouse
Rat
Chimpanzee
Rhesus monkey
Orangutan
Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 21,27 kDa (predicted molecular weight: 27 kDa).
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 21,27 kDa (predicted molecular weight: 27 kDa).

Target

  • Function

    Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP).

  • Tissue specificity

    Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes.

  • Domain

    The mature N-terminus mediates interaction with XIAP.

  • Cellular localization

    Mitochondrion. Released into the cytosol when cells undergo apoptosis.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 0610041G12Rik antibody
    • DBLOH_HUMAN antibody
    • DBOH antibody
    • DFNA64 antibody
    • diablo antibody
    • Diablo homolog (Drosophila) antibody
    • Diablo homolog antibody
    • Diablo homolog mitochondrial antibody
    • Diablo IAP binding mitochondrial protein antibody
    • Diablo like protein antibody
    • DIABLO S antibody
    • Direct IAP binding protein with low pI antibody
    • Direct IAP-binding protein with low pI antibody
    • FLJ10537 antibody
    • FLJ25049 antibody
    • mitochondrial antibody
    • Mitochondrial Smac protein antibody
    • Second mitochondria derived activator of caspase antibody
    • Second mitochondria-derived activator of caspase antibody
    • second mitochondrial activator of caspases antibody
    • SMAC 3 antibody
    • Smac antibody
    • Smac protein antibody
    • SMAC3 antibody
    see all

Images

  • Western blot - Anti-Smac/Diablo antibody (ab66290)
    Western blot - Anti-Smac/Diablo antibody (ab66290)
    All lanes : Anti-Smac/Diablo antibody (ab66290) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
    Lane 8 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 27 kDa
    Observed band size: 27 kDa
    Additional bands at: 21 kDa (possible isoform), 60 kDa, 75 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 10 minutes


    This antibody was raised against an immunogen that is predicted to cross react with both isoforms 1 and 2 of Smac/Diablo.

  • Western blot - Anti-Smac/Diablo antibody (ab66290)
    Western blot - Anti-Smac/Diablo antibody (ab66290)
    Anti-Smac/Diablo antibody (ab66290) at 1 µg/ml + Recombinant Human Smac / Diablo protein (ab87850) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 27 kDa
    Observed band size: 57 kDa
    why is the actual band size different from the predicted?


    Exposure time: 2 minutes

References (0)

Publishing research using ab66290? Please let us know so that we can cite the reference in this datasheet.

ab66290 has not yet been referenced specifically in any publications.

Images

  • Western blot - Anti-Smac/Diablo antibody (ab66290)
    Western blot - Anti-Smac/Diablo antibody (ab66290)
    All lanes : Anti-Smac/Diablo antibody (ab66290) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
    Lane 8 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 27 kDa
    Observed band size: 27 kDa
    Additional bands at: 21 kDa (possible isoform), 60 kDa, 75 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 10 minutes


    This antibody was raised against an immunogen that is predicted to cross react with both isoforms 1 and 2 of Smac/Diablo.

  • Western blot - Anti-Smac/Diablo antibody (ab66290)
    Western blot - Anti-Smac/Diablo antibody (ab66290)
    Anti-Smac/Diablo antibody (ab66290) at 1 µg/ml + Recombinant Human Smac / Diablo protein (ab87850) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 27 kDa
    Observed band size: 57 kDa
    why is the actual band size different from the predicted?


    Exposure time: 2 minutes

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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