Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FHL3 antibody [EPR14243(B)] - BSA and Azide free (ab250211)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14243(B)] to FHL3 - BSA and Azide free
  • Suitable for: ICC, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-FHL3 antibody [EPR14243(B)] - BSA and Azide free
    See all FHL3 primary antibodies

  • Description

    Rabbit monoclonal [EPR14243(B)] to FHL3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250211 is the carrier-free version of ab180509. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250211 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-FHL3 antibody [EPR14243(B)] - BSA and Azide free (ab250211)
    Western blot - Anti-FHL3 antibody [EPR14243(B)] - BSA and Azide free (ab250211)
    All lanes : Anti-FHL3 antibody [EPR14243(B)] (ab180509) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : Rat muscle tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 31 kDa



    This data was developed using ab180509, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry - Anti-FHL3 antibody [EPR14243(B)] - BSA and Azide free (ab250211)
    Immunocytochemistry - Anti-FHL3 antibody [EPR14243(B)] - BSA and Azide free (ab250211)
    This data was developed using ab180509, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of A673 cells labeling FHL3 with ab180509 at 1/100 dilution. Secondary ab: Goat anti rabbit IgG (Alexa Fluor® 555) at 1/250 dilution. Fixative: 4% paraformaldehyde. Counter stain: Dapi
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHL3 antibody [EPR14243(B)] - BSA and Azide free (ab250211)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHL3 antibody [EPR14243(B)] - BSA and Azide free (ab250211)
    This data was developed using ab180509, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human muscle tissue labeling FHL3 with ab180509 at 1/500 dilution. Counter stain: Hematoxylin Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Western blot - Anti-FHL3 antibody [EPR14243(B)] - BSA and Azide free (ab250211)
    Western blot - Anti-FHL3 antibody [EPR14243(B)] - BSA and Azide free (ab250211)
    Anti-FHL3 antibody [EPR14243(B)] (ab180509) at 1/5000 dilution + Human skeletal muscle lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG HRP (ab136636) at 1/500 dilution

    Predicted band size: 31 kDa



    This data was developed using ab180509, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Anti-FHL3 antibody [EPR14243(B)] - BSA and Azide free (ab250211)
    Anti-FHL3 antibody [EPR14243(B)] - BSA and Azide free (ab250211)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.