Anti-GM-CSF antibody [EPR23689-17] - BSA and Azide free (ab277945)
![Anti-GM-CSF antibody [EPR23689-17] - BSA and Azide free (ab277945)](https://www.abcam.com/ps/products/277/ab277945/Images/ab277945-1-anti-gmcsf-antibody-epr2368917-flow-cytometry-human-peripheral-blood-mononuclear-cell.jpg "Anti-GM-CSF antibody [EPR23689-17] - BSA and Azide free (ab277945)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23689-17] to GM-CSF - BSA and Azide free
- Suitable for: Flow Cyt
- Reacts with: Mouse, Human
Overview
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Product name
Anti-GM-CSF antibody [EPR23689-17] - BSA and Azide free
See all GM-CSF primary antibodies -
Description
Rabbit monoclonal [EPR23689-17] to GM-CSF - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Mouse
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow cyt: Human peripheral blood mononuclear cell (PBMC) treated with cell stimulation cocktail (80nM PMA+1.34uM Ionomycin+10.6uM Brefeldin A+2uM Monensin) for 6 hours; Mouse peripheral blood mononuclear cell (PBMC) treated with cell stimulation cocktail (80nM PMA+1.34uM Ionomycin+10.6uM Brefeldin A+2uM Monensin) for 6 hours.
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General notes
ab277945 is the carrier-free version of ab227031. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab277945 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23689-17 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-GM-CSF antibody [EPR23689-17] - BSA and Azide free (ab277945)
This data was developed using ab227031, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Human peripheral blood mononuclear cell (PBMC) treated with cell stimulation cocktail (80nM PMA+1.34uM Ionomycin+10.6uM Brefeldin A+2uM Monensin) for 6 hours cells labelling GM-CSF with ab227031 at 1/50 dilution Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were stained with anti-CD4 conjugated to Alexa Fluor® 647. Then fixed with 2% PFA followed by intracellularly stained with rabbit IgG or ab227031.
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![Flow Cytometry - Anti-GM-CSF antibody [EPR23689-17] - BSA and Azide free (ab277945)](https://www.abcam.com/ps/products/277/ab277945/Images/ab277945-2-anti-gmcsf-antibody-epr2368917-flow-cytometry-mouse-peripheral-blood-mononuclear-cell.jpg)
Flow Cytometry - Anti-GM-CSF antibody [EPR23689-17] - BSA and Azide free (ab277945)This data was developed using ab227031, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Mouse peripheral blood mononuclear cell (PBMC) treated with cell stimulation cocktail (80nM PMA+1.34uM Ionomycin+10.6uM Brefeldin A+2uM Monensin) for 6 hours cells labelling GM-CSF with ab227031 at 1/50 dilution Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were stained with anti-CD4 conjugated to Alexa Fluor® 647. Then fixed with 2% PFA followed by intracellularly stained with rabbit IgG or ab227031.
