Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] - BSA and Azide free (ab249987)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12998] to GABA Transporter 1 / GAT 1 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] - BSA and Azide free
    See all GABA Transporter 1 / GAT 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR12998] to GABA Transporter 1 / GAT 1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab249987 is the carrier-free version of ab177483. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab249987 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] - BSA and Azide free (ab249987)
    Western blot - Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] - BSA and Azide free (ab249987)
    All lanes : Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (ab177483) at 1/10000 dilution

    Lane 1 : Human brain lysates unboiled
    Lane 2 : Human brain lysates boiled
    Lane 3 : Mouse brain unboiled
    Lane 4 : Mouse brain boiled
    Lane 5 : Rat brain unboiled
    Lane 6 : Rat brain boiled

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 67 kDa
    Observed band size: 67 kDa



    This data was developed using ab177483, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-2: 20 seconds; Lanes 3-6: 8 seconds.

    We recommend not to boil the samples after lysis to get desired WB results.

  • Western blot - Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] - BSA and Azide free (ab249987)
    Western blot - Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] - BSA and Azide free (ab249987)
    Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (ab177483) at 1/1000 dilution + Fetal brain lysate at 10 µg

    Predicted band size: 67 kDa



    This data was developed using ab177483, the same antibody clone in a different buffer formulation.

     

  • Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] - BSA and Azide free (ab249987)
    Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] - BSA and Azide free (ab249987)

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