Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] - BSA and Azide free (ab249987)
![Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] - BSA and Azide free (ab249987)](https://www.abcam.com/ps/products/249/ab249987/Images/ab249987-403447-anti-gaba-transporter-1-gat-1-antibody-epr12998-bsa-and-azide-free-western-blot.jpg "Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] - BSA and Azide free (ab249987)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12998] to GABA Transporter 1 / GAT 1 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] - BSA and Azide free
See all GABA Transporter 1 / GAT 1 primary antibodies -
Description
Rabbit monoclonal [EPR12998] to GABA Transporter 1 / GAT 1 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab249987 is the carrier-free version of ab177483. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab249987 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12998 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] - BSA and Azide free (ab249987)All lanes : Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (ab177483) at 1/10000 dilution
Lane 1 : Human brain lysates unboiled
Lane 2 : Human brain lysates boiled
Lane 3 : Mouse brain unboiled
Lane 4 : Mouse brain boiled
Lane 5 : Rat brain unboiled
Lane 6 : Rat brain boiled
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 67 kDa
Observed band size: 67 kDaThis data was developed using ab177483, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times: Lanes 1-2: 20 seconds; Lanes 3-6: 8 seconds.
We recommend not to boil the samples after lysis to get desired WB results.
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![Western blot - Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] - BSA and Azide free (ab249987)](https://www.abcam.com/ps/products/249/ab249987/Images/ab249987-398862-anti-gaba-transporter-1-gat-1-antibody-epr12998-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] - BSA and Azide free (ab249987) -
![Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] - BSA and Azide free (ab249987)](https://www.abcam.com/ps/products/249/ab249987/Images/ab249987-3-benefits-of-recombinant-antibodies.png)
Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] - BSA and Azide free (ab249987)
