Human Von Willebrand Factor ELISA Kit (ab223864)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.079 ng/ml
- Range: 0.469 ng/ml - 30 ng/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human, Rhesus monkey
Overview
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Product name
Human Von Willebrand Factor ELISA Kit
See all Von Willebrand Factor kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 3 3.4% Inter-assay Sample n Mean SD CV% Serum 5 6.4% -
Sample type
Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.079 ng/ml -
Range
0.469 ng/ml - 30 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 101 94% - 107% Cell culture media 88 80% - 95% Hep Plasma 95 89% - 102% EDTA Plasma 92 86% - 97% Cit plasma 98 83% - 118% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human, Rhesus monkey
Does not react with: Cow -
Product overview
Human Von Willebrand Factor ELISA Kit (ab223864) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Von Willebrand Factor protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Von Willebrand Factor with 0.079 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Von Willebrand Factor is critical in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Von Willebrand Factor also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
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Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Human Von Willabrand Factor Capture Antibody (Lyophilized) 1 vial 10X Human Von Willabrand Factor Detector Antibody 1 x 600µl Human Von Willabrand Factor Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma. -
Tissue specificity
Plasma. -
Involvement in disease
Defects in VWF are the cause of von Willebrand disease (VWD) [MIM:277480]. VWD defines a group of hemorrhagic disorders in which the von Willebrand factor is either quantitatively or qualitatively abnormal resulting in altered platelet function. Symptoms vary depending on severity and disease type but may include prolonged bleeding time, deficiency of factor VIII and impaired platelet adhesion. Type I von Willebrand disease is the most common form and is characterized by partial quantitative plasmatic deficiency of an otherwise structurally and functionally normal Willebrand factor; type II is associated with a qualitative deficiency and functional anomalies of the Willebrand factor; type III is the most severe form and is characterized by total or near-total absence of Willebrand factor in the plasma and cellular compartments, also leading to a profound deficiency of plasmatic factor VIII. -
Sequence similarities
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 4 TIL (trypsin inhibitory-like) domains.
Contains 3 VWFA domains.
Contains 3 VWFC domains.
Contains 4 VWFD domains. -
Domain
The von Willebrand antigen 2 is required for multimerization of vWF and for its targeting to storage granules. -
Post-translational
modificationsAll cysteine residues are involved in intrachain or interchain disulfide bonds.
N- and O-glycosylated. -
Cellular localization
Secreted. Secreted > extracellular space > extracellular matrix. Localized to storage granules. - Information by UniProt
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Alternative names
- Coagulation factor VIII
- Coagulation factor VIII VWF
- F8VWF
see all -
Database links
- Entrez Gene: 7450 Human
- Omim: 613160 Human
- SwissProt: P04275 Human
- Unigene: 440848 Human
Images
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Other - Human Von Willebrand Factor ELISA Kit (ab223864)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Von Willebrand Factor standard curve in Sample Diluent NSBackground-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Von Willebrand Factor in human serum and plasma samplesThe concentrations of Von Willebrand Factor were measured in duplicates, interpolated from the Von Willebrand Factor standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:500, plasma (citrate) 1:500, plasma (heparin) 1:500, and plasma (EDTA) 1:500. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Von Willebrand Factor concentration was determined to be 4.226 µg/mL in neat serum, 2.793 µg/mL in neat plasma (citrate), 1.774 µg/mL in neat plasma (heparin), and 2.819 µg/mL in neat plasma (EDTA).
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Serum from ten individual healthy human female donors was measured in duplicateInterpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Von Willebrand Factor concentration was determined to be 5.280 µg/mL with a range of 2.088 – 11.35 µg/mL.
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Sandwich ELISA - Human Von Willebrand Factor ELISA Kit (ab223864)To learn more about the advantages of recombinant antibodies see here.
