Human Von Willebrand Factor ELISA Kit (VWF) (ab108918)
Key features and details
- Sensitivity: 0.38 mIU/ml
- Range: 0.625 mIU/ml - 40 mIU/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Von Willebrand Factor ELISA Kit (VWF)
See all Von Willebrand Factor kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 5.6% Inter-assay Sample n Mean SD CV% Overall 9.6% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
= 0.38 mIU/ml -
Range
0.625 mIU/ml - 40 mIU/ml -
Recovery
98 %
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Assay time
5h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human Von Willebrand Factor (VWF) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Human Von Willebrand Factor in plasma, serum, and cell culture supernatants.
A Von Willebrand Factor specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Von Willebrand Factor specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Von Willebrand Factor captured in plate.
Get higher sensitivity in only 90 minutes with Human Von Willebrand Factor ELISA Kit (ab189571) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Von Williebrand Factor Antibody 1 x 120µl Chromogen Substrate 1 x 7ml Sealing Tapes 3 units Stop Solution 1 x 11ml Von Williebrand Factor Microplate (12 x 8 well strips) 1 unit Von Williebrand Factor Standard 1 vial -
Research areas
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Function
Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma. -
Tissue specificity
Plasma. -
Involvement in disease
Defects in VWF are the cause of von Willebrand disease (VWD) [MIM:277480]. VWD defines a group of hemorrhagic disorders in which the von Willebrand factor is either quantitatively or qualitatively abnormal resulting in altered platelet function. Symptoms vary depending on severity and disease type but may include prolonged bleeding time, deficiency of factor VIII and impaired platelet adhesion. Type I von Willebrand disease is the most common form and is characterized by partial quantitative plasmatic deficiency of an otherwise structurally and functionally normal Willebrand factor; type II is associated with a qualitative deficiency and functional anomalies of the Willebrand factor; type III is the most severe form and is characterized by total or near-total absence of Willebrand factor in the plasma and cellular compartments, also leading to a profound deficiency of plasmatic factor VIII. -
Sequence similarities
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 4 TIL (trypsin inhibitory-like) domains.
Contains 3 VWFA domains.
Contains 3 VWFC domains.
Contains 4 VWFD domains. -
Domain
The von Willebrand antigen 2 is required for multimerization of vWF and for its targeting to storage granules. -
Post-translational
modificationsAll cysteine residues are involved in intrachain or interchain disulfide bonds.
N- and O-glycosylated. -
Cellular localization
Secreted. Secreted > extracellular space > extracellular matrix. Localized to storage granules. - Information by UniProt
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Alternative names
- Coagulation factor VIII
- Coagulation factor VIII VWF
- F8VWF
see all -
Database links
- Entrez Gene: 7450 Human
- Omim: 613160 Human
- SwissProt: P04275 Human
- Unigene: 440848 Human
Images
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Sandwich ELISA - Von Willebrand Factor (VWF) Human ELISA Kit (ab108918)
VWF measured in biological fluids with background signal subtracted (duplicates +/- SD). There is limited cross-reactivity in mouse (
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Human von Willebrand Factor ELISA Kit example standard curveExample von Willebrand Factor standard curve – data provided for demonstration purposes only.
