Anti-Cdc25B (phospho S323) antibody (ab53103)
Key features and details
- Rabbit polyclonal to Cdc25B (phospho S323)
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Cdc25B (phospho S323) antibody
See all Cdc25B primary antibodies -
Description
Rabbit polyclonal to Cdc25B (phospho S323) -
Host species
Rabbit -
Specificity
Detects endogenous levels of Cdc25B only when phosphorylated at serine 323 -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide derived from Human Cdc25B around the phosphorylation site of serine 323 (SPSPMP)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Cdc25B (phospho S323) antibody (ab53103)All lanes : Anti-Cdc25B (phospho S323) antibody (ab53103) at 1/300 dilution
Lane 1 : NIH/3T3 cell extract treated with PMA (125ng/ml, 30 mins)
Lane 2 : NIH/3T3 cell extract treated with PMA (125ng/ml, 30 mins) and the immunogenic phosphopeptide.
Predicted band size: 65 kDa
Observed band size: 65 kDa
