All lanes : Anti-Protein phosphatase 1 gamma 2 antibody [EPR8934] (ab134947) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : PPP1CC knockout HeLa cell lysate
Lane 3 : T-47D cell lysate
Lane 4 : 293T cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 37 kDa
Observed band size: 37 kDa

Lanes 1-4: Merged signal (red and green). Green - ab134947 observed at 37 kDa. Red - loading control ab7291 observed at 50 kDa.

ab134947 Anti-Protein phosphatase 1 gamma 2 antibody was shown to specifically react with Protein phosphatase 1 gamma 2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264875 (knockout cell lysate ab258131) was used. Wild-type and Protein phosphatase 1 gamma 2 knockout samples were subjected to SDS-PAGE. ab134947 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

 

Homozygous: Insertion of the selection cassette in exon 1.