Antibodies, Proteins, Kits and Reagents for Life Science

351 792 ₸ Product size

100 µl 351 792 ₸

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Anti-Smac/Diablo antibody [Y12] (ab32023)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [Y12] to Smac/Diablo
Suitable for: WB, IHC-P, IP, ICC/IF
Reacts with: Human

Product name

Anti-Smac/Diablo antibody [Y12]
See all Smac/Diablo primary antibodies
Description

Rabbit monoclonal [Y12] to Smac/Diablo
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human Smac/ Diablo (C terminal). The exact sequence is proprietary.
Epitope

ab32023 reacts with an epitope located in the C terminal region of Smac / Diablo.
Positive control
- WB: Jurkat whole cell lysate (ab7899). IHC-P: Human skin cancer tissue. ICC/IF: HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

Y12
Isotype

IgG
Research areas

Western blot - Anti-Smac/Diablo antibody [Y12] (ab32023)

Anti-Smac/Diablo antibody [Y12] (ab32023) at 1/1000 dilution + Jurkat cell lysate

Predicted band size: 27 kDa
Observed band size: 21 kDa
why is the actual band size different from the predicted?
Immunocytochemistry/ Immunofluorescence - Anti-Smac/Diablo antibody [Y12] (ab32023)

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling Smac / Diablo with ab32023 at 1/500. Cells were fixed with 100% methanol. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
Control: PBS only.
Nuclear counter stain: DAPI.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smac/Diablo antibody [Y12] (ab32023)

ab32023, at a 1/10 dilution, staining human Smac/Diablo in skin cancer tissue by immunohistochemistry, paraffin-embedded tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-Smac/Diablo antibody [Y12] (ab32023) Image from Lamers F et al., BMC Cancer. 2012 Jul 12;12:285. doi: 10.1186/1471-2407-12-285. Fig 3.; 12 July 2012, BMC Cancer 2012, 12:285

Immunofluorescence analysis of IMR32 cells, staining Smac / Diablo with ab32023. Cells were fixed with 4% paraformaldehyde before incubating with primary antibody. An Alexa Fluor^® conjugated anti-rabbit IgG was used as the secondary antibody.
Immunoprecipitation - Anti-Smac/Diablo antibody [Y12] (ab32023) This image is courtesy of an anonymous Abreview.

Smac / Diablo immunprecipitated from mitochondria isolated from mice brains.
Total protein in input: 800 µg.
Immunoprecipitation step: Dynabeads protein G.
Incubation: 16 hours, 4°C.
Diluent: TNE buffer.

For western blot, ab32023 was used at a 1/1000 dilution.

Lanes:
1. BioRad Precision Plus Protein standards, bands from top to bottom: 250, 150, 100, 75, 50, 37, 25, 20, 15, 10 kDa
2. Mitochondria isolated from mice brains, lysate
3. IP with anti-SMAC/DIABLO ab32023
See Abreview
Anti-Smac/Diablo antibody [Y12] (ab32023)

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