Anti-Smac/Diablo antibody (ab8114)
Key features and details
- Rabbit polyclonal to Smac/Diablo
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Smac/Diablo antibody
See all Smac/Diablo primary antibodies -
Description
Rabbit polyclonal to Smac/Diablo -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Peptide (
EERAESEQEAYLRED
)corresponding to amino acids 225 to 239 of humanSmac/DIABLO (1).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Smac Antibody is affinity chromatography purified via peptide column. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Smac/Diablo antibody (ab8114)All lanes : Anti-Smac/Diablo antibody (ab8114) at 1 µg/ml
Lane 1 : human heart tissue lysate with absence of blocking peptide
Lane 2 : human heart tissue lysate with Smac / Diablo peptide (ab8380)
Predicted band size: 25 kDa
Observed band size: 25 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smac/Diablo antibody (ab8114)Immunohistochemical analysis of paraffin-embedded human ovary tissue with ab8114 at 5 μg/mL. Sections were fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Western blot - Anti-Smac/Diablo antibody (ab8114)All lanes : Anti-Smac / Diablo antibody (ab8114) at 1 µg/mL, 1h incubation at RT in 5% NFM/TBST.
Loading: 15 μg of lysates per lane.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution. -
Western blot - Anti-Smac/Diablo antibody (ab8114)Anti-Smac/Diablo antibody (ab8114) at 1 µg/ml + NIH3T3 cell lysate at 15 µg
Secondary
Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 25 kDa
