Immunohistochemical analysis of paraffin-embedded Human breast adenocarcinoma tissue labeling Ki67 with ab156956 at 1/150 dilution. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min.

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Ki67 with ab156956 at 1/150 dilution. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min.

Overlay histogram showing Ramos cells stained with ab156956 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab156956, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Ramos cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Immunohistochemical analysis of paraffin-embedded Human colon adenocarcinoma tissue labeling Ki67 with ab156956 at 1/150 dilution. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min.

Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling Ki67 with ab156956 at 1/150 dilution. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min.

Immunohistochemical analysis of paraffin-embedded Human endometrium adenocarcinoma tissue labeling Ki67 with ab156956 at 1/150 dilution. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min.

Immunohistochemical analysis of paraffin-embedded Human bladder carcinoma tissue labeling Ki67 with ab156956 at 1/150 dilution. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min.

Immunohistochemical analysis of paraffin-embedded Human lymphoma tissue labeling Ki67 with ab156956 at 1/150 dilution. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min.

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Ki67 with ab156956 at 1/150 dilution. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min.