Anti-CD44 antibody [KM81] (ab112178)
Key features and details
- Rat monoclonal [KM81] to CD44
- Suitable for: IHC-P, Flow Cyt
- Reacts with: Mouse
- Isotype: IgG2a
Overview
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Product name
Anti-CD44 antibody [KM81]
See all CD44 primary antibodies -
Description
Rat monoclonal [KM81] to CD44 -
Host species
Rat -
Tested applications
Suitable for: IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Mouse Bone Marrow Derived Stromal Cells (clone BMS2).
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Positive control
- Mouse Bone Marrow cells (BALB/c strain). IHC-P: Mouse lung FFPE tissue sections.
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General notes
This product was changed from ascites to tissue culture supernatant on 21st December 2017. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
ab112178 is purified from ascitic fluid via Protein G Chromatography. -
Clonality
Monoclonal -
Clone number
KM81 -
Myeloma
Sp2/0 -
Isotype
IgG2a -
Research areas
Images
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ab112178, at 0.2 µg/106 cells, staining CD44 in Mouse Bone Marrow cells (strain: BALB/c) by Flow cytometry.
Percentage of cells stained above control: 98.1%. -
IHC image of CD44 staining in mouse lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab112178, 1µg/ml, for 15 mins at room temperature. A goat anti-rat biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.