Anti-PTEN antibody [SP227] (ab227674)
![Anti-PTEN antibody [SP227] (ab227674)](https://www.abcam.com/ps/products/227/ab227674/Images/ab227674-3-AntiPTEN-antibody-SP227-IHC-Human-tonsil.jpg "Anti-PTEN antibody [SP227] (ab227674)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP227] to PTEN
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-PTEN antibody [SP227]
See all PTEN primary antibodies -
Description
Rabbit monoclonal [SP227] to PTEN -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Mouse
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human tonsil tissue; WB: Staurosporine treated NIH/3T3 cell lysate.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP227 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [SP227] (ab227674)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling PTEN with ab227674 at 1/100 dilution (1.48 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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![Western blot - Anti-PTEN antibody [SP227] (ab227674)](https://www.abcam.com/ps/products/227/ab227674/Images/ab227674-290524-anti-pten-antibody-sp227-western-blot.jpg)
Western blot - Anti-PTEN antibody [SP227] (ab227674)All lanes : Anti-PTEN antibody [SP227] (ab227674) at 1/400 dilution
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) treated with staurosporine, cell lysate
Lane 2 : Untreated NIH/3T3 cell lysate
Developed using the ECL technique.
Predicted band size: 47 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [SP227] (ab227674)](https://www.abcam.com/ps/products/227/ab227674/Images/ab227674-290525-anti-pten-antibody-sp227-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [SP227] (ab227674)Formalin-fixed, paraffin-embedded human tonsil tissue stained for PTEN with ab227674 at 1/100 dilution in immunohistochemical analysis.
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![Anti-PTEN antibody [SP227] (ab227674)](https://www.abcam.com/ps/products/227/ab227674/Images/ab227674-4-benefits-of-recombinant-antibodies.png)
Anti-PTEN antibody [SP227] (ab227674)
