Anti-PP2A alpha + beta antibody (ab137849)
Key features and details
- Rabbit polyclonal to PP2A alpha + beta
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PP2A alpha + beta antibody
See all PP2A alpha + beta primary antibodies -
Description
Rabbit polyclonal to PP2A alpha + beta -
Host species
Rabbit -
Specificity
ab137849 does cross react with PP2A beta -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Chicken, Cow, Dog, Pig, Xenopus laevis, Zebrafish, Xenopus tropicalis -
Immunogen
Synthetic peptide corresponding to Human PP2A-alpha aa 247-259.
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Positive control
- 293T, A431, H1299, HeLaS3, Molt4, Raji and mouse brain lysates; HeLa cell; A549 xenograft
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 89.99% PBS, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-PP2A alpha + beta antibody (ab137849) at 1/1500 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : Molt4 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 35 kDa
10% SDS PAGE -
Anti-PP2A alpha + beta antibody (ab137849) at 1/10000 dilution + mouse brain whole cell lysate at 50 µg
Predicted band size: 35 kDa
10% SDS PAGE -
Confocal immunofluorescence analysis of paraformaldehyde fixed HeLa cell labeling PP2A alpha with ab137849 (green) at a 1/500 dilution. Alpha tubulin filaments were labeled with an Alpha tubulin antibody (red) at a 1/2000 dilution.
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Immunohistochemical analysis of paraffin embedded A549 xenograft, labeling PP2A alpha using ab137849 at a 1/100 dilution.