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271 382 ₸ Product size

7 ml 271 382 ₸

Order now and get it on Wednesday March 17, 2021

Anti-PTEN antibody [SP218] - C-terminal, prediluted (ab228467)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [SP218] to PTEN - C-terminal, prediluted
Suitable for: IHC-P
Reacts with: Human

Product name

Anti-PTEN antibody [SP218] - C-terminal, prediluted
See all PTEN primary antibodies
Description

Rabbit monoclonal [SP218] to PTEN - C-terminal, prediluted
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Human prostate adenocarcinoma and colon adenocarcinoma tissues.
General notes

This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: Tris buffered saline, 1% BSA
Concentration information loading...
Purity

Protein A/G purified
Purification notes

Purified from TCS by protein A/G.
Clonality

Monoclonal
Clone number

SP218
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [SP218] - C-terminal, prediluted (ab228467)

Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for PTEN using ab228467 in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [SP218] - C-terminal, prediluted (ab228467)

Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for PTEN using ab228467 in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [SP218] - C-terminal, prediluted (ab228467)

Cancer cells from prostate adenocarcinoma (panel A) and colon adenocarcinoma (panel D) show total loss or diminished expression of PTEN when SP218 is used. In contrast, nuclear staining (panel B and C, arrows) in cancer cells is present for clones D4.3 and 138G6. Diffuse cytoplasmic and stromal staining are prominent for clones D4.3 and 138G6. But interestingly, the stromal cell components (fibroblasts, lymphocytes, and endothelial cells) are stained with similar intensities across all images. This suggests that there is non-specific staining in stromal and cancer area with clones D4.3 and 138G6 when compared with SP218.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [SP218] - C-terminal, prediluted (ab228467)

Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for PTEN using ab228467 in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [SP218] - C-terminal, prediluted (ab228467)

Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for PTEN using ab228467 in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [SP218] - C-terminal, prediluted (ab228467)

Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for PTEN using ab228467 in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [SP218] - C-terminal, prediluted (ab228467)

Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for PTEN using ab228467 in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [SP218] - C-terminal, prediluted (ab228467)

Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for PTEN using ab228467 in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [SP218] - C-terminal, prediluted (ab228467)

Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for PTEN using ab228467 in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [SP218] - C-terminal, prediluted (ab228467)

Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for PTEN using ab228467 in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [SP218] - C-terminal, prediluted (ab228467)

Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for PTEN using ab228467 in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [SP218] - C-terminal, prediluted (ab228467)

Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for PTEN using ab228467 in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [SP218] - C-terminal, prediluted (ab228467)

Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for PTEN using ab228467 in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [SP218] - C-terminal, prediluted (ab228467)

Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for PTEN using ab228467 in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [SP218] - C-terminal, prediluted (ab228467)

Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for PTEN using ab228467 in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [SP218] - C-terminal, prediluted (ab228467)

Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for PTEN using ab228467 in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [SP218] - C-terminal, prediluted (ab228467)

Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for PTEN using ab228467 in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [SP218] - C-terminal, prediluted (ab228467)

Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for PTEN using ab228467 in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [SP218] - C-terminal, prediluted (ab228467)

Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for PTEN using ab228467 in immunohistochemical analysis.
Anti-PTEN antibody [SP218] - C-terminal, prediluted (ab228467)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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