Anti-Cdc25A (phospho S18) antibody [20HCLC] (ab277771)
![Anti-Cdc25A (phospho S18) antibody [20HCLC] (ab277771)](https://www.abcam.com/ps/products/277/ab277771/Images/ab277771-2-antiPhosphoCdc25AS18recombinantpolyclonalantibody20hclcWB.jpg "Anti-Cdc25A (phospho S18) antibody [20HCLC] (ab277771)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit recombinant multiclonal [20HCLC] to Cdc25A (phospho S18)
- Suitable for: ICC, WB
- Reacts with: Human
Overview
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Product name
Anti-Cdc25A (phospho S18) antibody [20HCLC]
See all Cdc25A primary antibodies -
Description
Rabbit recombinant multiclonal [20HCLC] to Cdc25A (phospho S18) -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide aa 14-21 (phospho S18).
Database link: P30304 -
Positive control
- WB: HCT116 and HeLa whole cell extracts (+/- UV treatment). ICC: HeLa cells (UV treated).
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General notes
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains.
Recombinant multiclonal antibodies offer the sensitivity of polyclonal antibodies by recognising multiple epitopes, along with consistency of a recombinant antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituent: 99.91% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Recombinant Multiclonal -
Clone number
20HCLC -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Cdc25A antibody (ab277771)All lanes :
Lane 1 : HCT116 whole cell extract
Lane 2 : HCT116 whole cell extract + UV treatment
Lane 3 : HeLa whole cell extract
Lane 4 : HeLa whole cell extract + UV treatment
Lane 5 : HeLa whole cell extract + UV + Nocodazole treatment
Predicted band size: 59 kDa
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Immunocytochemistry - Anti-Cdc25A antibody (ab277771)Immunofluorescence was performed on fixed and permeabilized HeLa cells treated with UV (302nM) for 60 minutes for detection of Cdc25apS18 using Anti-Cdc25apS18 Recombinant Rabbit Multiclonal Antibody (ab277771, 2 μ/mL) and labeled with Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate (1/2000). Panel a) shows representative cells that were stained for detection and localization of Cdc25apS18 protein (green), Panel b) is stained for nuclei (blue) using DAPI. Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (1/300). Panel d) is a composite image of Panels a, b and c clearly demonstrating cytoplasmic and nuclear localization of Cdc25apS18. Panel e) represents composite image of untreated cells with no signal. Panel f) represents control cells with no primary antibody to assess background.
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![Anti-Cdc25A antibody [20HCLC] (ab277771)](https://www.abcam.com/ps/products/277/ab277771/Images/ab277771-3-benefits-of-recombinant-antibodies.jpg)
Anti-Cdc25A antibody [20HCLC] (ab277771)