Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LATS2 antibody [EPR23126-488] - BSA and Azide free (ab280363)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23126-488] to LATS2 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-LATS2 antibody [EPR23126-488] - BSA and Azide free
    See all LATS2 primary antibodies

  • Description

    Rabbit monoclonal [EPR23126-488] to LATS2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Recombinant fragment
    WB
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa and HEK-293T whole cell lysates; His-tagged human LATS2 recombinant protein. IP: HeLa whole cell lysate.

  • General notes

    ab280363 is the carrier-free version of ab243657. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab280363 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-LATS2 antibody [EPR23126-488] - BSA and Azide free (ab280363)
    Western blot - Anti-LATS2 antibody [EPR23126-488] - BSA and Azide free (ab280363)
    All lanes : Anti-LATS2 antibody [EPR23126-488] (ab243657) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : HEK-293T (human embryonic kidney epithelial cell), whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 120 kDa
    Observed band size: 140 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab243657, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Fresh lysates were used in this WB.

    Exposure time: 3 minutes

  • Immunoprecipitation - Anti-LATS2 antibody [EPR23126-488] - BSA and Azide free (ab280363)
    Immunoprecipitation - Anti-LATS2 antibody [EPR23126-488] - BSA and Azide free (ab280363)

    This data was developed using ab243657, the same antibody clone in a different buffer formulation.

    LATS2 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate with ab243657 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab243657 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate 10 ug

    Lane 2: ab243657 IP in HeLa whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab243657 in HeLa whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 64 seconds

    Fresh lysates were used in this WB.

  • Western blot - Anti-LATS2 antibody [EPR23126-488] - BSA and Azide free (ab280363)
    Western blot - Anti-LATS2 antibody [EPR23126-488] - BSA and Azide free (ab280363)
    All lanes : Anti-LATS2 antibody [EPR23126-488] (ab243657) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), transfected with scrambled siRNA control, whole cell lysate
    Lane 2 : HeLa transfected with siRNA specifically targeting LATS2, whole cell lysate 20

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 120 kDa
    Observed band size: 140 kDa why is the actual band size different from the predicted?



    This data was developed using ab243657, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Fresh lysates were used in this WB.

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure time: 3 minutes

     

  • Western blot - Anti-LATS2 antibody [EPR23126-488] - BSA and Azide free (ab280363)
    Western blot - Anti-LATS2 antibody [EPR23126-488] - BSA and Azide free (ab280363)
    All lanes : Anti-LATS2 antibody [EPR23126-488] (ab243657) at 1/1000 dilution

    Lane 1 : His-tagged human LATS1 recombinant protein (aa701-1054)
    Lane 2 : His-tagged human LATS2 recombinant protein (aa664-1016)

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 120 kDa



    This data was developed using ab243657, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 3 minutes

     

  • Anti-LATS2 antibody [EPR23126-488] - BSA and Azide free (ab280363)
    Anti-LATS2 antibody [EPR23126-488] - BSA and Azide free (ab280363)

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