Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] - BSA and Azide free (ab251404)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16968(B)] to ACTR1B + ACTR1A - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] - BSA and Azide free
    See all ACTR1B + ACTR1A primary antibodies

  • Description

    Rabbit monoclonal [EPR16968(B)] to ACTR1B + ACTR1A - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251404 is the carrier-free version of ab203833. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251404 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Clonality

    Monoclonal

  • Clone number

    EPR16968(B)

  • Isotype

    IgG

Images

  • Western blot - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] - BSA and Azide free (ab251404)
    Western blot - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] - BSA and Azide free (ab251404)
    All lanes : Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] (ab203833) at 1/1000 dilution

    Lane 1 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
    Lane 2 : MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate
    Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


    Exposure time: 3 minutes


    This data was developed using ab203833, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] - BSA and Azide free (ab251404)
    Western blot - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] - BSA and Azide free (ab251404)
    All lanes : Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] (ab203833) at 1/1000 dilution

    Lane 1 : Human fetal brain tissue lysate
    Lane 2 : Human fetal kidney tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


    Exposure time: 3 minutes


    This data was developed using ab203833, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] - BSA and Azide free (ab251404)
    Western blot - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] - BSA and Azide free (ab251404)
    Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] (ab203833) at 1/1000 dilution + Full length Human ACTR1A recombination protein at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


    Exposure time: 1 second


    This data was developed using ab203833, the same antibody clone in a different buffer formulation.

  • Immunoprecipitation - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] - BSA and Azide free (ab251404)
    Immunoprecipitation - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] - BSA and Azide free (ab251404)
    This data was developed using ab203833, the same antibody clone in a different buffer formulation.ACTR1B + ACTR1A was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate with ab203833 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab203833 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1500. Lane 1: Jurkat whole cell lysate 10ug (Input). Lane 2: ab203833 IP in Jurkat whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203833 in Jurkat whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 10 seconds.
  • Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] - BSA and Azide free (ab251404)
    Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] - BSA and Azide free (ab251404)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.