Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Podoplanin / gp36 antibody [LpMab-7] (ab256561)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [LpMab-7] to Podoplanin / gp36
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Podoplanin / gp36 antibody [LpMab-7]
    See all Podoplanin / gp36 primary antibodies

  • Description

    Mouse monoclonal [LpMab-7] to Podoplanin / gp36

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Tissue, cells or virus. This information is considered to be commercially sensitive.

  • Positive control

    • WB: U-2 OS cell lysate. IHC-P: Human mesothelioma, Human placenta tissues.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Podoplanin / gp36 antibody [LpMab-7] (ab256561)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Podoplanin / gp36 antibody [LpMab-7] (ab256561)

    Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling Podoplanin / gp36 with ab256561 at 1/10000 dilution, detected using Leica DS9800 (Bond™ Polymer Refine Detection). Positive staining on the vessels in the human placenta. The section was incubated with ab256561 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Leica DS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Podoplanin / gp36 antibody [LpMab-7] (ab256561)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Podoplanin / gp36 antibody [LpMab-7] (ab256561)

    Immunohistochemical analysis of paraffin-embedded Human mesothelioma tissue labeling Podoplanin / gp36 with ab256561 at 1/10000 dilution, detected using Leica DS9800 (Bond™ Polymer Refine Detection). Membranous staining on human mesothelioma. The section was incubated with ab256561 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Leica DS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Western blot - Anti-Podoplanin / gp36 antibody [LpMab-7] (ab256561)
    Western blot - Anti-Podoplanin / gp36 antibody [LpMab-7] (ab256561)
    All lanes : Anti-Podoplanin / gp36 antibody [LpMab-7] (ab256561) at 1/1000 dilution

    Lane 1 : U-2 OS (human bone osteosarcoma epithelial cell), whole cell lysate
    Lane 2 : HUVEC (human umbilical vein endothelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 16 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST The molecular weight observed is consistent with what has been described in the literature (PMID: 25080943; PMID: 26090595; PMID: 26416352).

    Negative control: HUVEC (PMID: 21920451)

    Exposure time: 20 seconds

  • Anti-Podoplanin / gp36 antibody [LpMab-7] (ab256561)
    Anti-Podoplanin / gp36 antibody [LpMab-7] (ab256561)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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