Anti-Amylin/DAP antibody (ab77387)
Key features and details
- Rabbit polyclonal to Amylin/DAP
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Amylin/DAP antibody
See all Amylin/DAP primary antibodies -
Description
Rabbit polyclonal to Amylin/DAP -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
Synthetic peptide corresponding to Human Amylin/DAP aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab103557) -
Positive control
- This antibody gave a positive signal in a human pancreas tissue section.
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General notes
This product was previously labelled as Amylin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab77387 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species IHC-P HumanAll applications RabbitCatDogBaboonApplication Abreviews Notes IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.Notes IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.Target
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Function
Selectively inhibits insulin-stimulated glucose utilization and glycogen deposition in muscle, while not affecting adipocyte glucose metabolism. -
Sequence similarities
Belongs to the calcitonin family. -
Domain
The mature protein is largely unstructured in the absence of a cognate ligand, and has a strong tendency to form fibrillar aggregates. Homodimerization may be the first step of amyloid formation. -
Post-translational
modificationsAmyloid fibrils are degraded by IDE. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 751513 Cat
- Entrez Gene: 3375 Human
- Entrez Gene: 100009489 Rabbit
- Omim: 147940 Human
- SwissProt: P12967 Cat
- SwissProt: P10997 Human
- SwissProt: Q07334 Rabbit
- Unigene: 46835 Human
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Alternative names
- Amylin antibody
- DAP antibody
- Diabetes associated peptide antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amylin/DAP antibody (ab77387)
IHC image of Amylin/DAP staining in Human Pancreas section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab77387, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
Datasheets and documents
References (0)
ab77387 has not yet been referenced specifically in any publications.
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amylin/DAP antibody (ab77387)
IHC image of Amylin/DAP staining in Human Pancreas section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab77387, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
