Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-AMPS antibody [EPR10747(B)] - BSA and Azide free (ab249082)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10747(B)] to AMPS - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-AMPS antibody [EPR10747(B)] - BSA and Azide free
    See all AMPS primary antibodies

  • Description

    Rabbit monoclonal [EPR10747(B)] to AMPS - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab249082 is the carrier-free version of ab154182. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab249082 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPS antibody [EPR10747(B)] - BSA and Azide free (ab249082)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPS antibody [EPR10747(B)] - BSA and Azide free (ab249082)

    This data was developed using ab154182, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human thyroid gland carcinoma tissue labeling AMPS with ab154182 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPS antibody [EPR10747(B)] - BSA and Azide free (ab249082)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPS antibody [EPR10747(B)] - BSA and Azide free (ab249082)

    This data was developed using ab154182, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin embedded Human normal brain tissue using ab154182 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Flow Cytometry - Anti-AMPS antibody [EPR10747(B)] - BSA and Azide free (ab249082)
    Flow Cytometry - Anti-AMPS antibody [EPR10747(B)] - BSA and Azide free (ab249082)

    This data was developed using ab154182, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of permeabilized HeLa cells with ab154182 at 1/100 dilution(red) or a rabbit IgG (negative) (green).
  • Western blot - Anti-AMPS antibody [EPR10747(B)] - BSA and Azide free (ab249082)
    Western blot - Anti-AMPS antibody [EPR10747(B)] - BSA and Azide free (ab249082)
    All lanes : Anti-AMPS antibody [EPR10747(B)] (ab154182) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : HCT-116 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 55 kDa



    This data was developed using ab154182, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPS antibody [EPR10747(B)] - BSA and Azide free (ab249082)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPS antibody [EPR10747(B)] - BSA and Azide free (ab249082)
    This data was developed using ab154182, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human endometrial carcinoma tissue using ab154182 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPS antibody [EPR10747(B)] - BSA and Azide free (ab249082)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPS antibody [EPR10747(B)] - BSA and Azide free (ab249082)
    This data was developed using ab154182, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human normal kidney tissue using ab154182 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPS antibody [EPR10747(B)] - BSA and Azide free (ab249082)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPS antibody [EPR10747(B)] - BSA and Azide free (ab249082)
    This data was developed using ab154182, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue using ab154182 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Anti-AMPS antibody [EPR10747(B)] - BSA and Azide free (ab249082)
    Anti-AMPS antibody [EPR10747(B)] - BSA and Azide free (ab249082)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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