Anti-PDHX antibody (ab232798)
Key features and details
- Rabbit polyclonal to PDHX
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-PDHX antibody
See all PDHX primary antibodies -
Description
Rabbit polyclonal to PDHX -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human, Pig -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human PDHX aa 200-500. (Expressed in E.coli).
Database link: O00330 -
Positive control
- WB: Recombinant human PDHX protein; A431 and HeLa cell lysates; Pig heart lysate. ICC/IF: HeLa cells. IHC-P: Human stomach cancer, breast cancer, prostate cancer, prostate, stomach and bile duct cancer tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PDHX antibody (ab232798)Anti-PDHX antibody (ab232798) at 3 µg/ml + Recombinant human PDHX protein
Secondary
HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 54 kDa
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Western blot - Anti-PDHX antibody (ab232798)All lanes : Anti-PDHX antibody (ab232798) at 3 µg/ml
Lane 1 : A431 (human epidermoid carcinoma cell line) cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : Pig heart lysate
Secondary
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 54 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHX antibody (ab232798)Formalin-fixed, paraffin-embedded human stomach cancer tissue stained for PDHX using ab232798 at 10 μg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHX antibody (ab232798)Formalin-fixed, paraffin-embedded human breast cancer tissue stained for PDHX using ab232798 at 10 μg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHX antibody (ab232798)Formalin-fixed, paraffin-embedded human prostate cancer tissue stained for PDHX using ab232798 at 10 μg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHX antibody (ab232798)Formalin-fixed, paraffin-embedded human prostate tissue stained for PDHX using ab232798 at 10 μg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHX antibody (ab232798)Formalin-fixed, paraffin-embedded human stomach tissue stained for PDHX using ab232798 at 10 μg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHX antibody (ab232798)Formalin-fixed, paraffin-embedded human bile duct cancer tissue stained for PDHX using ab232798 at 10 μg/ml in immunohistochemical analysis. DAB staining.
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Immunocytochemistry/ Immunofluorescence - Anti-PDHX antibody (ab232798)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for PDHX (green) using ab232798 at 20 μg/ml in ICC/IF. FITC-labeled secondary antibody.
