Recombinant Human SGT1/ECD protein (ab211952)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His-T7 tag N-Terminus
- Suitable for: SDS-PAGE
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Product name
Recombinant Human SGT1/ECD protein -
Purity
> 90 % SDS-PAGE.
This protein was expressed in E. coli as inclusion bodies, refolded using unique “temperature shift inclusion body refolding” technology and chromatographically purified. -
Expression system
Escherichia coli -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MASMTGGQQMGRGHHHHHHENLYFQGGEFELEETMKLATMEDTVEYCLFL IPDESRDSDKHKEILQKYIERIITRFAPMLVPYIWQNQPFNLKYKPGKGG VPAHMFGVTKFGDNIEDEWFIVYVIKQITKEFPELVARIEDNDGEFLLIE AADFLPKWLDPENSTNRVFFCHGELCIIPAPRKSGAESWLPTTPPTIPQA LNIITAHSEKILASESIRAAVNRRIRGYPEKIQASLHRAHCFLPAGIVAV LKQRPRLVAAAVQAFYLRDPIDLRACRVFKTFLPETRIMTSVTFTKCLYA QLVQQRFVPDRRSGYRLPPPSDPQYRAHELGMKLAHGFEILCSKCSPHFS DCKKSLVTASPLWASFLESLKKNDYFKGLIEGSAQYRERLEMAENYFQLS VDWPESSLAMSPGEEILTLLQTIPFDIEDLKKEAANLPPEDDDQWLDLSP DQLDQLLQEAVGKKESESVSKEEKEQNYDLTEVSESMKAFISKVSTHKGA ELPREPSEAPITFDADSFLNYFDKILGPRPNESDSDDLDDEDFECLDSDD DLDFETHEPGEEASLKGTLDNLKSYMAQMDQELAHTCISKSFTTRNQVEP VSQTTDNNSDEEDSGTGESVMAPVDVDLNLVSNILESYSSQAGLAGPASN LLQSMGVQLPDNTDHRPTSKPTKN -
Predicted molecular weight
76 kDa including tags -
Amino acids
2 to 644 -
Tags
His-T7 tag N-Terminus -
Additional sequence information
Constructed with 31 aa N-terminal T7 / His / TEV cleavage site Tag. NP_009196.
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Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituent: 0.32% Tris HCl
Contains NaCl, KCl, EDTA, Sucrose, DTT.