Citrate Assay Kit (ab83396)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 0.002 mM
Overview
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Product name
Citrate Assay Kit -
Detection method
Colorimetric/Fluorometric -
Sample type
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts -
Assay type
Quantitative -
Sensitivity
> 0.002 mM -
Range
0.002 mM - 10 mM -
Assay time
0h 40m -
Product overview
Citrate Assay Kit ab83396 provides a simple, sensitive and rapid means of quantifying citrate in biological samples.
In the citrate assay protocol, citrate is converted to pyruvate via oxaloacetate. The pyruvate is quantified by converting a nearly colorless probe to an intensely colored (570 nm) and fluorescent (Ex/Em, 535/587 nm) product.
The citrate assay kit can detect 0.1 to 10 nmoles (~2 µM-10 mM) of citrate.
Citrate assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min at room temp
- analyze with microplate reader -
Notes
Citric acid (HOOC-CH2-C(-OH)(-COOH)-CH2-COOH) is a key intermediate in the TCA cycle which occurs in mitochondria. It is formed by the addition of oxaloacetate to the acetyl group of acetyl-CoA derived from the glycolytic pathway. Citrate can be transported out of mitochondria and converted back to acetyl CoA for fatty acid synthesis.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Citrate Assay Buffer WM 1 x 25ml Citrate Developer (Lyophilised) Green 1 vial Citrate Enzyme Mix (Lyophilised) Purple 1 vial Citrate Probe Red 1 x 0.2ml Citrate Standard (10 µmol) (lyophilised) Yellow 1 vial -
Research areas
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Relevance
Citric acid (HOOC-CH2-C(-OH)(-COOH)-CH2-COOH) is a key intermediate in the TCA cycle which occurs in mitochondria. It is formed by the addition of oxaloacetate to the acetyl group of acetyl-CoA derived from the glycolytic pathway. Citrate can be transported out of mitochondria and converted back to acetyl CoA for fatty acid synthesis. Citrate is an allosteric modulator of both fatty acid synthesis (acetyl-CoA carboxylase) and glycolysis (phospho-fructokinase). Citrate is widely used industrially in foods, beverages and pharmaceuticals. Citrate metabolism and disposition can vary widely due to sex, age and a variety of other factors. -
Alternative names
- Citric acid
Images
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Functional assay - 83396 Citrate Assay Kit
Fluorimetric standard curve: mean of duplicates (+/-SD) with background readings subtracted.
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Functional assay - 83396 Citrate Assay KitCitrate measured fluorimetrically in various biofluids showing concentration (micromolar).
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Functional Studies - Citrate Assay Kit (ab83396)Citrate standard curve generated using this kit protocol