Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NDUFB10 antibody [EPR16230-47] (ab196019)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16230-47] to NDUFB10
  • Suitable for: ICC/IF, IP, IHC-P, WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-NDUFB10 antibody [EPR16230-47]
    See all NDUFB10 primary antibodies

  • Description

    Rabbit monoclonal [EPR16230-47] to NDUFB10

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, HepG2 and Jurkat cell lysates; Human transitional cell carcinoma of bladder tissue; HeLa cells; HeLa whole cell extract.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-NDUFB10 antibody [EPR16230-47] (ab196019)
    Western blot - Anti-NDUFB10 antibody [EPR16230-47] (ab196019)
    All lanes : Anti-NDUFB10 antibody [EPR16230-47] (ab196019) at 1/10000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma) cell lysate
    Lane 3 : Jurkat (Human T cell leukemia cells from peripheral blood) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 21 kDa
    Observed band size: 21 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB10 antibody [EPR16230-47] (ab196019)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB10 antibody [EPR16230-47] (ab196019)

    Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling NDUFB10 with ab196019 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on Human transitional cell carcinoma of bladder tissue is observed. Counter stained with Hematoxylin.

    Secondary control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-NDUFB10 antibody [EPR16230-47] (ab196019)
    Immunocytochemistry/ Immunofluorescence - Anti-NDUFB10 antibody [EPR16230-47] (ab196019)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling NDUFB10 with ab196019 at 1/350 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on HeLa cell line is observed. The nuclear counter stain is DAPI (blue).

  • Flow Cytometry - Anti-NDUFB10 antibody [EPR16230-47] (ab196019)
    Flow Cytometry - Anti-NDUFB10 antibody [EPR16230-47] (ab196019)

    Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labelling NDUFB10 (red) with purified ab196019 at a dilution of 1/800. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary and secondary antibody.

  • Immunoprecipitation - Anti-NDUFB10 antibody [EPR16230-47] (ab196019)
    Immunoprecipitation - Anti-NDUFB10 antibody [EPR16230-47] (ab196019)

    NDUFB10 was immunoprecipitated from HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab196019 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab196019 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: HeLa whole cell extract 10 µg (Input). Lane 2: ab196019 IP in HeLa whole cell extract. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab196019 in HeLa whole cell extract.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

  • Anti-NDUFB10 antibody [EPR16230-47] (ab196019)
    Anti-NDUFB10 antibody [EPR16230-47] (ab196019)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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