Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Pancreatic Polypeptide antibody [EPR23320-10] (ab272732)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23320-10] to Pancreatic Polypeptide
  • Suitable for: IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Pancreatic Polypeptide antibody [EPR23320-10]
    See all Pancreatic Polypeptide primary antibodies

  • Description

    Rabbit monoclonal [EPR23320-10] to Pancreatic Polypeptide

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human, mouse and rat pancreas tissue. IHC-Fr: Mouse and rat pancreas tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23320-10

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Frozen sections) - Anti-Pancreatic Polypeptide antibody [EPR23320-10] (ab272732)
    Immunohistochemistry (Frozen sections) - Anti-Pancreatic Polypeptide antibody [EPR23320-10] (ab272732)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat pancreas tissue labeling Pancreatic Polypeptide with ab272732 at 1/1000 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 µg/ml) dilution (Green). Positive staining on PP cells in rat pancreatic islet is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 µg/ml) dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Frozen sections) - Anti-Pancreatic Polypeptide antibody [EPR23320-10] (ab272732)
    Immunohistochemistry (Frozen sections) - Anti-Pancreatic Polypeptide antibody [EPR23320-10] (ab272732)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse pancreas tissue labeling Pancreatic Polypeptide with ab272732 at 1/1000 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 µg/ml) dilution (Green). Positive staining on PP cells in mouse pancreatic islet is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 µg/ml) dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pancreatic Polypeptide antibody [EPR23320-10] (ab272732)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pancreatic Polypeptide antibody [EPR23320-10] (ab272732)

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling Pancreatic Polypeptide with ab272732 at 1/2000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on PP cells of rat pancreas. The section was incubated with ab272732 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pancreatic Polypeptide antibody [EPR23320-10] (ab272732)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pancreatic Polypeptide antibody [EPR23320-10] (ab272732)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling Pancreatic Polypeptide with ab272732 at 1/2000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on PP cells of mouse pancreas (PMID: 27270601). The section was incubated with ab272732 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pancreatic Polypeptide antibody [EPR23320-10] (ab272732)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pancreatic Polypeptide antibody [EPR23320-10] (ab272732)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Pancreatic Polypeptide with ab272732 at 1/2000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on PP cells of human pancreas. The section was incubated with ab272732 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-Pancreatic Polypeptide antibody [EPR23320-10] (ab272732)
    Anti-Pancreatic Polypeptide antibody [EPR23320-10] (ab272732)

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