Human Triiodothyronine ELISA Kit (free + total T3) (ab108664)
Key features and details
- Sensitivity: 0.2 ng/ml
- Range: 0.2 ng/ml - 10 ng/ml
- Sample type: Serum
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
Overview
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Product name
Human Triiodothyronine ELISA Kit (free + total T3)
See all Triiodothyronine (T3) kits -
Detection method
Colorimetric -
Sample type
Serum -
Assay type
Competitive -
Sensitivity
= 0.2 ng/ml -
Range
0.2 ng/ml - 10 ng/ml -
Recovery
106 %
Sample specific recovery Sample type Average % Range Serum 106 0.68ng/ml - 5.81ng/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Triiodothyronine (T3) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Triiodothyronine (T3) in Human serum.
A 96-well plate has been pre-coated with goat anti-mouse IgG. Samples, standards and the mouse anti-Triiodothyronine Reagent and Triiodothyronine-HRP conjugate are added to the wells, where Triiodothyronine in the sample competes with the added Triiodothyronine-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Estradiol E2 in the sample and the intensity is measured at 450 nm.
This kit detects total and free T3. To detect only free T3 please use ab108663.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 11X Triiodothyronine HRP Conjugate 1 x 1.3ml Goat anti-mouse IgG Coated Microplate (12 x 8 wells) 1 x 96 tests HRP-Conjugate Diluent 1 x 13ml Stop Solution 1 x 11ml T3 Antibody Reagent 1 x 7ml T3 Standard 0 - 0 ng/mL 1 x 1ml T3 Standard 1 - 0.75 ng/mL 1 x 1ml T3 Standard 2 - 1.5 ng/mL 1 x 1ml T3 Standard 3 - 3 ng/mL 1 x 1ml T3 Standard 4 - 6 ng/mL 1 x 1ml T3 Standard 5 - 10 ng/mL 1 x 1ml TMB Reagent 1 x 11ml -
Research areas
Images
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Typical Standard Curve
Representative standard curve using ab108664
