Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DRAK2 antibody [EPR3163Y] - BSA and Azide free (ab201323)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3163Y] to DRAK2 - BSA and Azide free
  • Suitable for: ICC, IP, WB
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-DRAK2 antibody [EPR3163Y] - BSA and Azide free
    See all DRAK2 primary antibodies

  • Description

    Rabbit monoclonal [EPR3163Y] to DRAK2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IP, WBmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Jurkat and Ramos cell lysates

  • General notes

    ab201323 is the carrier-free version of ab108373 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab201323 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-DRAK2 antibody [EPR3163Y] - BSA and Azide free (ab201323)
    Western blot - Anti-DRAK2 antibody [EPR3163Y] - BSA and Azide free (ab201323)

    This data was developed using ab108373, the same antibody clone in a different buffer formulation.

    Lane 1 Wild-type HAP1 cell lysate (20 µg)

    Lane 2 DRAK2 knockout HAP1 cell lysate (20 µg)

    Lane 3 Ramos cell lysate (20 µg)

    Lane 4 Raji cell lysate (20 µg)

    Lanes 1 - 4 Merged signal (red and green). Green - ab108373 observed at 42 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab108373 was shown to recognize DRAK2 when DRAK2 knockout samples were used, along with additional cross-reactive bands. Wild-type and DRAK2 knockout samples were subjected to SDS-PAGE. ab108373 and ab8245 (loading control to GAPDH) were diluted 1/500 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD)preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • Immunocytochemistry - Anti-DRAK2 antibody [EPR3163Y] - BSA and Azide free (ab201323)
    Immunocytochemistry - Anti-DRAK2 antibody [EPR3163Y] - BSA and Azide free (ab201323)
    This data was developed using ab108373, the same antibody clone in a different buffer formulation.Immunofluorescence staining of Ramos cells with purified ab108373 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab108373 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
  • Western blot - Anti-DRAK2 antibody [EPR3163Y] - BSA and Azide free (ab201323)
    Western blot - Anti-DRAK2 antibody [EPR3163Y] - BSA and Azide free (ab201323)
    All lanes : Anti-DRAK2 antibody [EPR3163Y] (ab108373) at 1/1000 dilution (purified)

    Lane 1 : C6 whole cell lysate
    Lane 2 : PC-12 whole cell lysate
    Lane 3 : NIH/3T3 whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa



    This data was developed using ab108373, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-DRAK2 antibody [EPR3163Y] - BSA and Azide free (ab201323)
    Western blot - Anti-DRAK2 antibody [EPR3163Y] - BSA and Azide free (ab201323)
    All lanes : Anti-DRAK2 antibody [EPR3163Y] (ab108373) at 1/10000 dilution (purified)

    Lane 1 : Ramos whole cell lysate
    Lane 2 : Raji whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa



    This data was developed using ab108373, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-DRAK2 antibody [EPR3163Y] - BSA and Azide free (ab201323)
    Immunoprecipitation - Anti-DRAK2 antibody [EPR3163Y] - BSA and Azide free (ab201323)
    This data was developed using ab108373, the same antibody clone in a different buffer formulation.ab108373 (purified) at 1/40 immunoprecipitating DRAK2 in 10 μg Ramos cell lysate (Lanes 1 and 2, observed at 42 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730). For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution. Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
  • Western blot - Anti-DRAK2 antibody [EPR3163Y] - BSA and Azide free (ab201323)
    Western blot - Anti-DRAK2 antibody [EPR3163Y] - BSA and Azide free (ab201323)
    All lanes : Anti-DRAK2 antibody [EPR3163Y] (ab108373) at 1/500 dilution (Unpurified)

    Lane 1 : Jurkat cell lysate +TPA
    Lane 2 : Ramos cell lysate
    Lane 3 : Ramos cell lysate + TPA

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 42 kDa



    This data was developed using ab108373, the same antibody clone in a different buffer formulation.

     

  • Anti-DRAK2 antibody [EPR3163Y] - BSA and Azide free (ab201323)
    Anti-DRAK2 antibody [EPR3163Y] - BSA and Azide free (ab201323)

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