Triiodothyronine (T3) ELISA Kit (ab108685)
Key features and details
- Sensitivity: 0.05 ng/ml
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
Overview
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Product name
Triiodothyronine (T3) ELISA Kit
See all Triiodothyronine (T3) kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 40 Inter-assay Sample n Mean SD CV% Overall 3 -
Sample type
Serum, Hep Plasma, Cit plasma -
Assay type
Competitive -
Sensitivity
0.05 ng/ml -
Recovery
97.5 %
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Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Triiodothyronine (T3) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of total Triiodothyronine in serum and plasma.
A 96-well plate has been precoated with anti-T3. Samples and the T3-HRP conjugate are added to the wells, where Triiodothyronine in the sample competes with the addedT3-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Triiodothyronine in the sample and the intensity is measured at 450 nm.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 11X T3-HRP Conjugate 1 x 1.4ml 50X Wash Solution 1 x 20ml Anti-T3 IgG Coated Microplate (12 x 8 wells) 1 unit Conjugate Buffer 1 x 12.5ml Cover foils 1 unit Stop Solution 1 x 15ml Strip holder 1 unit T3 Standard 0 - 0.0 ng/mL 1 x 1ml T3 Standard 1 - 0.5 ng/mL 1 x 1ml T3 Standard 2 - 1.0 ng/mL 1 x 1ml T3 Standard 3 - 2.5 ng/mL 1 x 1ml T3 Standard 4 - 5.0 ng/mL 1 x 1ml T3 Standard 5 - 7.5 ng/mL 1 x 1ml TMB Substrate Solution 1 x 15ml -
Research areas
Images
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Typical Standard Curve
Representative Standard Curve using ab108685
